Proliferation in monocyte-derived dendritic cell cultures is caused by progenitor cells capable of myeloid differentiation
Proliferation in monocyte-derived dendritic cell cultures is caused by progenitor cells capable of myeloid differentiation
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DOI：
10.1182/blood.v92.5.1598.417k33_1598_1607
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发表时间：
1998-09-01
期刊：
影响因子：
20.3
通讯作者：
Thomas, R
中科院分区：
文献类型：
--
作者：
Cavanagh, LL;Saal, RJ;Thomas, R
树突状细胞（DC）可以通过在粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-4（IL-4）存在下培养粘附的外周血（PB）细胞来产生。对于这些DC是来自增殖前体细胞还是仅仅来自单核细胞的分化存在争议。从骨髓富集的PB非T细胞或分选的单核细胞产生DC。从任一群体产生的DC充当有效的抗原呈递细胞。在从骨髓富集的非T细胞培养的DC中观察到[H-3]-胸苷的摄取。添加脂多糖或肿瘤坏死因子-α导致DC成熟，但不抑制增殖。在这些DC的细胞涂片中观察到Ki 67（+）细胞，通过双重染色，它们是CD 3（-）CD 19（-）CD 11 c（-）CD 40（-）和髓过氧化物酶（+），表明它们是髓样祖细胞。通过流式细胞术对起始群体的分析表明，在标准测定中产生了少量的CD 34（+）CD 33（-）CD 14（-）祖细胞和大量的粒细胞-巨噬细胞集落形成单位。因此，从贴壁PB细胞体外产生DC也富集了暴露于GM-CSF后能够增殖的祖细胞。具有临床重要性的是，在GM-CSF和IL-4存在下衍生的DC的产量不能扩增超过起始单核细胞的数量。(C)1998年，美国血液学会。
Dendritic cells (DC) can be generated by culture of adherent peripheral blood (PB) cells in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). There is controversy as to whether these DC arise from proliferating precursors or simply from differentiation of monocytes. DC were generated from myeloid-enriched PB non-T cells or sorted monocytes. DC generated from either population functioned as potent antigen-presenting cells. Uptake of [H-3]-thymidine was observed in DC cultured from myeloid-enriched non-T cells. Addition of lipopolysaccharide or tumor necrosis factor-alpha led to maturation of the DC, but did not inhibit proliferation. Ki67(+) cells were observed in cytospins of these DC, and by double staining were CD3(-)CD19(-)CD11c(-)CD40(-) and myeloperoxidase(+), suggesting that they were myeloid progenitor cells. Analysis of the starting population by flow cytometry demonstrated small numbers of CD34(+)CD33(-)CD14(-) progenitor cells, and numerous granulocyte-macrophage colony-forming units were generated in standard assays. Thus, production of DC in vitro from adherent PB cells also enriches for progenitor cells that are capable of proliferation after exposure to GM-CSF. Of clinical importance, the yield of DC derived in the presence of GM-CSF and IL-4 cannot be expanded beyond the number of starting monocytes. (C) 1998 by The American Society of Hematology.