Flower extracts from Paeonia decomposita and Paeonia ostii inhibit melanin synthesis via cAMP-CREB-associated melanogenesis signaling pathways in murine B16 melanoma cells
Flower extracts from Paeonia decomposita and Paeonia ostii inhibit melanin synthesis via cAMP-CREB-associated melanogenesis signaling pathways in murine B16 melanoma cells
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DOI：
10.1111/jfbc.12777
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发表时间：
2019-04-01
影响因子：
4
通讯作者：
Zheng, Xuxu
中科院分区：
文献类型：
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作者：
Guo, Lixia;Yin, Zhongyi;Zheng, Xuxu
本研究旨在确定不同花部分的EtOAc级分的分解（Pd）和口叶番荔枝（Po）对黑素生成的影响及其在B16黑色素瘤细胞中的作用机制。细胞活力测定表明，Pd-1、Po-1（Pd或Po的花瓣）、Pd-3和Po-3（Pd或Po的雄蕊）在25 μ g/ml时对B16细胞的毒性较低。Pd-1和Po-1提取物显著降低黑色素含量，抑制酪氨酸酶和多巴氧化酶活性。此外，Pd-1和Po-1下调MC1R、MITF、TRP-1、TRP-2和酪氨酸酶的表达。这些提取物还降低cAMP水平并抑制CREB的磷酸化，这可能是由于存在高浓度的酚类化合物和黄酮类化合物。我们的研究结果表明，Pd-1和Po-1能够调节cAMP-CREB信号通路，并下调黑素生成相关蛋白，从而产生所观察到的抗黑素生成作用。实际应用在中国，红毛菊的花经常被用作膳食补充剂和皮肤美白产品的添加剂。2013年11月，中华人民共和国国家卫生和计划生育委员会已批准将乌藨子花作为一种新的食品资源。本研究首次证实，裂叶番荔枝（Pd）和口叶番荔枝（Po）花瓣的乙酸乙酯组分可显著降低B16细胞中黑色素含量，其机制可能是通过调节cAMP-CREB信号通路，下调黑素生成相关蛋白的表达。Pd和Po提取物作为天然酪氨酸酶抑制剂，可作为食品添加剂、化妆品材料甚至治疗色素沉着疾病的良好候选物。
This investigation seeks to identify the effects of the EtOAc fractions of different flower parts of Paeonia decomposita (Pd) and Paeonia ostii (Po) on melanogenesis and their mechanisms of action in B16 melanoma cells. Cell viability assay showed that Pd-1, Po-1 (the petals of Pd or Po), Pd-3 and Po-3 (the stamens of Pd or Po) at 25 mu g/ml produced lower toxic activities in B16 cells. Pd-1 and Po-1 extracts considerably reduced the melanin content and inhibited tyrosinase and DOPA oxidase activity. Moreover, Pd-1 and Po-1 down-regulated the expressions of MC1R, MITF, TRP-1, TRP-2, and tyrosinase. These extracts also reduce cAMP levels and inhibited the phosphorylation of CREB, which might be due to the presence of high concentrations of phenolic compounds and flavonoids. Our results suggested that Pd-1 and Po-1 are able to modulate the cAMP-CREB signaling pathway and down-regulate the melanogenesis-related proteins resulting in the observed anti-melanogenic effects. Practical applications In China, the flower of Paeonia is often consumed as a dietary supplement and as an additive in skin whitening products. In November 2013, the National Health and Family Planning Commission of the People's Republic of China has approved the flower of Paeonia ostii as a novel food resource. The current study firstly demonstrated that the effects of EtOAc fractions of the petals of Paeonia decomposita (Pd) and Paeonia ostii (Po) considerably reduced the melanin content in B16 cells, which is due to the modulation of the cAMP-CREB signaling pathway followed by the down-regulation of melanogenesis-related proteins. Pd and Po extracts, as natural tyrosinase inhibitors, may serve as good candidates in food additives, cosmetic materials, or even in treating hyperpigmentation diseases.