SYNERGISTIC EFFECT OF ACUTE-HYPOXIA ON FLOW-INDUCED RELEASE OF ATP FROM CULTURED ENDOTHELIAL-CELLS
SYNERGISTIC EFFECT OF ACUTE-HYPOXIA ON FLOW-INDUCED RELEASE OF ATP FROM CULTURED ENDOTHELIAL-CELLS
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DOI：
10.1007/bf01931108
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发表时间：
1995-03-15
期刊：
影响因子：
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通讯作者：
BURNSTOCK, G
中科院分区：
文献类型：
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作者：
BODIN, P;BURNSTOCK, G
将原代培养的人脐静脉内皮细胞（HUVECs）在常氧或低氧条件下灌注。通过增加流速（从0.5至3.0 ml/min）刺激这些细胞两次，持续3 min。在低氧条件下，ATP的基础释放与常氧条件下相同，但在流量增加时释放更多（0.58 +/- 0.07 > 0.32 +/- 0.04 pmoles/ml/10（6）个细胞（+78%），对于第一刺激期; 0.39 +/- 0.05 > 0.22 +/- 0.03 pmoles/ml/10（6）个细胞（+79%）。进一步的流量递增实验表明，在常氧和低氧条件下，ATP释放量与流量呈正相关，但无论流量如何，低氧条件下ATP释放量均高于原代培养的HUVEC。在常氧和低氧条件下没有观察到差异。在这两种情况下，高流量期间释放的ATP量（0.050 +/- 0.004 pmoles/ml/10（6）cells）显著小于低流量期间释放的ATP量（0.09 +/-0.01 pmoles/ml/10（6）cells）。综上所述，由于缺氧单独不影响ATP释放，在刺激HUVEC释放ATP中，增加的剪切应力和缺氧之间似乎存在协同关系。此外，在这些条件下释放ATP似乎是高度分化的内皮细胞的特性。
Human umbilical vein endothelial cells (HUVECs) in primary cultures were perfused under normoxic or hypoxic conditions. These cells were stimulated twice for 3 min by increased flow (from 0.5 to 3.0 ml/min). Under hypoxic conditions the basal release of ATP was the same as under normoxic conditions, but during increased flow the release was greater (0.58 +/- 0.07 > 0.32 +/- 0.04 pmoles/ml/10(6) cells ( +78%), for the first period of stimulation; 0.39 +/- 0.05 > 0.22 +/- 0.03 pmoles/ml/10(6) cells ( +79%) for the second period). Further experiments with sequential increments in flow rate showed that under both normoxic and hypoxic conditions, a positive correlation existed between ATP release and the rate of flow but there was always more ATP released under hypoxic conditions regardless of the flow rate.HUVECs in secondary culture (second passage) were similarly stimulated. No differences were observed between normoxic and hypoxic conditions. In both cases, the quantity of ATP released during high flow (0.050 +/- 0.004 pmoles/ml/10(6) cells) was significantly smaller than the quantity of ATP released during low flow (0.09 +/- 0.01 pmoles/ml/10(6) cells).To conclude, since hypoxia alone did not affect ATP release, there appears to be a synergistic relationship between increased shear stress and hypoxia in the stimulation of ATP release from HUVECs. Moreover, the release of ATP under these conditions seems to be a property of highly differentiated endothelial cells.