ISOLATION AND CHARACTERIZATION OF FRUIT VACUOLAR INVERTASE GENES FROM 2 TOMATO SPECIES AND TEMPORAL DIFFERENCES IN MESSENGER-RNA LEVELS DURING FRUIT RIPENING
ISOLATION AND CHARACTERIZATION OF FRUIT VACUOLAR INVERTASE GENES FROM 2 TOMATO SPECIES AND TEMPORAL DIFFERENCES IN MESSENGER-RNA LEVELS DURING FRUIT RIPENING
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DOI：
10.1007/bf00028808
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发表时间：
1993-02-01
影响因子：
5.1
通讯作者：
MIRKOV, TE
中科院分区：
文献类型：
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作者：
ELLIOTT, KJ;BUTLER, WO;MIRKOV, TE
以番茄栽培种番茄基因组克隆和果实液泡转化酶基因为材料，研究成熟番茄果实转化酶基因表达与葡萄糖和果糖积累的关系。UC82B和野生种番茄(Lycopsicpimpinellifolium)进行了分离鉴定。所有被检测的克隆的编码序列都是相同的。与已知的雪莲果实液泡转化酶的氨基酸序列进行比较，发现该氨基酸序列中含有一个可能的信号序列，以及一个可能的氨基端和羧基末端的前肽。其中42%的残基与胡萝卜细胞壁转化酶的残基一致。在番茄序列中，胡萝卜、酵母和细菌转化酶中也存在一个假定的催化位点和一个五个残基的基序。两种番茄基因组克隆的核苷酸序列在一个内含子和推测的调控区有微小的差异。该基因似乎存在于每个单倍体基因组的一个拷贝中。Northern分析表明，这两个物种在果实发育过程中液泡转化酶mRNA水平的时间模式不同，野生物种的转化酶mRNA出现在果实发育的早期阶段。在推测的调控区域中发现的核苷酸差异可能与该基因的时间调控的物种差异有关，这反过来可能有助于观察到成熟果实中己糖积累的差异。
To determine the relationship between invertase gene expression and glucose and fructose accumulation in ripening tomato fruit, fruit vacuolar invertase cDNA and genomic clones from the cultivated species, Lycopersicon esculentum cv. UC82B, and a wild species, Lycopersicon pimpinellifolium, were isolated and characterized. The coding sequences of all cDNA clones examined are identical. By comparison to the known amino acid sequence of mature L. esculentum fruit vacuolar invertase, a putative signal sequence and putative amino-terminal and carboxy-terminal propeptides were identified in the derived amino acid sequence. Of the residues 42% are identical with those of carrot cell wall invertase. A putative catalytic site and a five-residue motif found in carrot, yeast, and bacterial invertases are also present in the tomato sequence. Minor differences between the nucleotide sequences of the genomic clones from the two tomato species were found in one intron and in the putative regulatory region. The gene appears to be present in one copy per haploid genome. Northern analysis suggests a different temporal pattern of vacuolar invertase mRNA levels during fruit development in the two species, with the invertase mRNA appearing at an earlier stage of fruit development in the wild species. Nucleotide differences found in the putative regulatory regions may be involved in species differences in temporal regulation of this gene, which in turn may contribute to observed differences in hexose accumulation in ripening fruit.