Inhibition of nitric oxide synthase blocks osteoclastic bone resorption in adaptive bone modeling.
Inhibition of nitric oxide synthase blocks osteoclastic bone resorption in adaptive bone modeling.
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DOI：
10.1080/00016489850183223
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发表时间：
1998-09
影响因子：
1.4
通讯作者：
R. Chole;S. Tinling;E. Leverentz;M. D. McGinn
中科院分区：
文献类型：
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作者：
R. Chole;S. Tinling;E. Leverentz;M. D. McGinn
在这项研究中，沙土鼠的听泡加压，导致积极的造型的骨泡壁的破骨细胞表面和矿物质沉积率显着增加。全身注射一氧化氮合酶（NOS）抑制剂L-N（G）-硝基精氨酸甲酯（L-NAME）可抑制该造模过程。与加压生理盐水处理的大疱相比，L-NAME处理的动物内表面大疱壁上的破骨细胞表面百分比（OC.S/BS）显著降低。荧光骨表面（BSf）矿物沉积率（MAR）和骨形成率（BFR）没有显着差异时，L-NAME组与对照组相比，加压大疱。然而，L-NAME显着抑制BSf在未加压的大疱。因此，一氧化氮很可能是通过一种或多种NOS亚型的适应性骨建模引起的骨吸收的介质。
In this study, the auditory bulla of the gerbil was pressurized, leading to active modeling of the bone of the bulla wall with a significant increase in osteoclast surface and mineral apposition rate. Systemic infusion of L-N(G)-nitro-arginine-methyl ester (L-NAME), an inhibitor of nitric oxide synthase (NOS), inhibited this modeling process. The percentage osteoclast surface (Oc.S/BS) on the inner surface bulla wall was significantly reduced in the L-NAME-treated animals when compared with pressurized saline-treated bullae. Fluorescent bone surface (BSf) mineral apposition rates (MAR) and bone formation rate (BFR) were not significantly different in the pressurized bullae when the L-NAME group was compared with the control (vehicle only) group. However, L-NAME significantly suppressed BSf in the unpressurized bullae. Therefore, it is likely that nitric oxide is a mediator of osteoclastic resorption due to adaptive bone modeling through one or more of the isoforms of NOS.