Constructing an adult orofacial premotor atlas in Allen mouse CCF.
Constructing an adult orofacial premotor atlas in Allen mouse CCF.
复制标题
在艾伦小鼠CCF中构建成人口面前运动图谱。
作者：
Takatoh J;Park JH;Lu J;Li S;Thompson PM;Han BX;Zhao S;Kleinfeld D;Friedman B;Wang F
脑干中的前运动回路投射到口面部运动神经元群，以执行诸如舔、咀嚼、呼吸等基本运动行为，在啮齿动物中还包括 whisking（拂动胡须，此处可能指动物用胡须探索环境的行为）。先前的跨突触追踪研究仅绘制了新生小鼠的口面前运动回路，但由于技术困难，成年动物的回路仍不为人知。在此，我们开发了一种三步单突触跨突触追踪策略，以识别控制成年小鼠触须、舌头伸出和下颌闭合肌肉的前运动神经元。我们将这些不同组的前运动神经元登记到艾伦小鼠大脑通用坐标框架（CCF）上，进而生成了一个组合的3D口面前运动图谱，揭示了不同前运动回路独特的空间组织。我们进一步发现了同时支配多个运动核的前运动神经元，因此它们可能协调参与相同口面运动行为的不同肌肉。我们追踪成年前运动回路并登记到艾伦CCF的方法具有普遍适用性，应该有助于对多种行为的运动控制进行研究。
Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors.