The influence of intermittent hypoxia, obesity, and diabetes on male genitourinary anatomy and voiding physiology
The influence of intermittent hypoxia, obesity, and diabetes on male genitourinary anatomy and voiding physiology
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DOI：
10.1152/ajprenal.00112.2021
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发表时间：
2021-07-01
影响因子：
4.2
通讯作者：
Vezina, Chad M.
中科院分区：
文献类型：
--
作者：
Abler, Lisa L.;O'Driscoll, Chelsea A.;Vezina, Chad M.
我们使用携带Lep(Ob)突变的雄性BTBR小鼠，这些小鼠在6周大时开始出现严重的进行性肥胖和糖尿病，以检查睡眠呼吸暂停的一种特殊表现--间歇性低氧(IH)对男性排尿生理和生殖器解剖的影响。用自制的装置连续给野生型和Lep(ob/ob)(突变)小鼠连续给予常氧(对照)或IH，持续2wk。在12h的非活动(光)期间，吸入90 S的6%氧气，然后注入90 S的室内空气。在12小时的活动(黑暗)期间，持续的室内空气输送。然后我们评估了泌尿生殖系统的解剖学和生理学。正如对2型糖尿病表型的预期一样，突变小鼠消耗了更多的食物和水，体重更重，排尿更频繁，尿量更大。与野生型小鼠相比，它们的膀胱体积更大，但前列腺、精囊和尿路更小。IH减少了突变小鼠的食物消耗，增加了不依赖于基因的膀胱相对重量，并增加了尿糖浓度。根据基因(Normooxia+IH)进行评估时，突变小鼠的病原性菌尿发生率高于野生型小鼠，而在IH暴露的小鼠中，突变小鼠的菌尿发生率高于野生型小鼠。我们的结论是，接触IH和2型糖尿病可以单独或共同作用于改变雄性小鼠的尿液功能。值得注意的新陈代谢综合征和阻塞性睡眠呼吸暂停在老年男性中很常见，两者都与尿排尿功能障碍有关。在这里，我们表明代谢综合征和间歇性低氧(睡眠呼吸暂停的一种表现)对雄性小鼠的排尿功能和泌尿生殖器官解剖有单独和联合的影响。
We used male BTBR mice carrying the Lep(ob) mutation, which are subject to severe and progressive obesity and diabetes beginning at 6 wk of age, to examine the influence of one specific manifestation of sleep apnea, intermittent hypoxia (IH), on male urinary voiding physiology and genitourinary anatomy. A custom device was used to deliver continuous normoxia (control) or IH to wild-type and Lep(ob/ob) (mutant) mice for 2 wk. IH was delivered during the 12-h inactive (light) period in the form of 90 s of 6% O-2 followed by 90 s of room air. Continuous room air was delivered during the 12-h active (dark) period. We then evaluated genitourinary anatomy and physiology. As expected for the type 2 diabetes phenotype, mutant mice consumed more food and water, weighed more, and voided more frequently and in larger urine volumes. They also had larger bladder volumes but smaller prostates, seminal vesicles, and urethras than wild-type mice. IH decreased food consumption and increased bladder relative weight independent of genotype and increased urine glucose concentration in mutant mice. When evaluated based on genotype (normoxia + IH), the incidence of pathogenic bacteriuria was greater in mutant mice than in wild-type mice, and among mice exposed to IH, bacteriuria incidence was greater in mutant mice than in wild-type mice. We conclude that IH exposure and type 2 diabetes can act independently and together to modify male mouse urinary function.NEW & NOTEWORTHY Metabolic syndrome and obstructive sleep apnea are common in aging men, and both have been linked to urinary voiding dysfunction. Here, we show that metabolic syndrome and intermittent hypoxia (a manifestation of sleep apnea) have individual and combined influences on voiding function and urogenital anatomy in male mice.