ANTIGENIC VARIANT OF SWINE INFLUENZA-VIRUS CAUSING PROLIFERATIVE AND NECROTIZING PNEUMONIA IN PIGS
ANTIGENIC VARIANT OF SWINE INFLUENZA-VIRUS CAUSING PROLIFERATIVE AND NECROTIZING PNEUMONIA IN PIGS
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DOI：
10.1177/104063879200400403
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发表时间：
1992-10-01
影响因子：
1.5
通讯作者：
MARTINEAU, GP
中科院分区：
文献类型：
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作者：
DEA, S;BILODEAU, R;MARTINEAU, GP
从魁北克7个不同猪群中出现呼吸道疾病的猪的肺中分离出一种新的猪流感病毒抗原变异体。不同日龄的猪均有发病，主要临床症状为发热、呼吸困难和腹式呼吸。咳嗽并不是该综合征的常见症状。尸检时，肉眼可见病变包括动物总体外观苍白、全身淋巴结病、肝充血和肺实变。组织学检查结果主要为增殖性肺炎伴显著巨噬细胞浸润、肺泡和气道中坏死的炎性细胞、II型肺细胞显著增殖和肺泡隔增厚。荧光抗体检查病猪肺部没有发现猪细小病毒，传染性胃肠炎病毒，或脑心肌炎病毒。然而，通过使用针对人A型流感病毒核衣壳蛋白（NP）的单克隆抗体进行间接免疫荧光（IIF）染色，证明存在A型流感病毒感染的证据。通过尿囊内接种无特定病原体的鸡胚或在胰蛋白酶存在下在犬肾（MDCK）细胞中增殖来分离病毒。通过血凝抑制试验，证实与人流感H1 N1、H2 N2和H3 N2毒株无交叉反应性，并且通过IIF，感染的MDCK细胞与抗B型流感病毒NP蛋白的单克隆抗体无反应。对猪流感病毒A/威斯康星州/76/H1 N1和A/新泽西/76/H1 N1亚型产生的超免疫血清仅部分抑制空斑纯化分离物的血凝活性。用这种猪流感病毒新的抗原变异体的蛋适应分离株鼻内感染的无菌仔猪发生了与田间病例中观察到的完全相同类型的病变。
A new antigenic variant of swine influenza virus was isolated from the lungs of pigs experiencing respiratory problems in 7 different swine herds in Quebec. Pigs of different ages were affected, and the main clinical signs were fever, dyspnea, and abdominal respiration. Coughing was not a constant finding of the syndrome. At necropsy, macroscopic lesions included the overall appearance of pale animals, general lymphadenopathy, hepatic congestion, and consolidation of the lungs. Histopathologic findings were mainly proliferative pneumonia with a significant macrophage invasion, necrotic inflammatory cells in the alveoli and the airways, a marked proliferation of type II pneumocytes, and thickening of the alveolar septae. Fluorescent antibody examination of lungs of sick piglets did not demonstrate porcine parvovirus, transmissible gastroenteritis virus, or encephalomyocarditis virus. However, evidence of the presence of an influenza type A infection was demonstrated by indirect immunofluorescence (IIF) staining using monoclonal antibody directed to nucleocapsid protein (NP) of human type A influenza virus. The virus was isolated either by intra-allantoic inoculation of specific-pathogen-free embryonating hens' eggs or propagation in canine kidney (MDCK) cells in the presence of trypsin. By hemagglutination inhibition tests, no cross-reactivity was demonstrated with human influenza H1N1, H2N2, and H3N2 strains, and infected MDCK cells did not react by IIF with monoclonal antibodies to NP protein of type B influenza virus. The hemagglutination activity of plaque-purified isolates was only partly inhibited by hyperimmune serum produced to subtypes A/Wisconsin/76/H1N1 and A/New Jersey/76/H1N1 of swine influenza virus. Gnotobiotic piglets that were infected intranasally with egg-adapted isolates of this new antigenic variant of swine influenza virus developed the very same type of lesions observed in field cases.