LncRNA TUG1 affects cell viability by regulating glycolysis in osteosarcoma cells
LncRNA TUG1 affects cell viability by regulating glycolysis in osteosarcoma cells
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DOI：
10.1016/j.gene.2018.06.085
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发表时间：
2018-10-20
期刊：
影响因子：
3.5
通讯作者：
Yang, Yiyong
中科院分区：
文献类型：
--
作者：
Han, Xiufu;Yang, Yanming;Yang, Yiyong
骨肉瘤是一种侵袭性的恶性肿瘤，好发于青少年和年轻人。长链非编码RNA（lncRNA）牛磺酸上调基因1（TUG 1）被认为是骨肉瘤的癌基因。然而，TUG 1在骨肉瘤中的调节机制尚未完全了解。我们的目的是研究代谢的改变是否参与了TUG 1对骨肉瘤细胞的作用。在此，我们发现TUG 1在骨肉瘤细胞中过表达，与正常成骨细胞系相比。TUG 1的敲除抑制骨肉瘤细胞的葡萄糖消耗、乳酸产生和细胞活力。TUG 1的过表达诱导细胞活力，而2-DG的诱导减弱。TUG 1的异常表达显著影响己糖激酶2（HK 2）的表达。HK 2的敲除减弱了TUG 1过表达对骨肉瘤细胞糖酵解的影响。我们的结论是糖酵解参与了TUG 1对骨肉瘤细胞活力的影响。HK 2可能是TUG 1影响糖酵解的重要分子。
Osteosarcoma is an aggressive malignant neoplasm in teenagers and young adults. Long non-coding RNA (lncRNA) taurine-upregulated gene 1 (TUG1) is considered as an oncogene in osteosarcoma. However, the mechanism of TUG1 in regulating osteosarcoma has not been fully understood. We aimed to investigate whether the metabolic alteration is involved in the effect of TUG1 on osteosarcoma cells. Herein, we found that TUG1 was overexpressed in osteosarcoma cells compared with the normal osteoblastic cell line. Knockdown of TUG1 inhibited glucose consumption, lactate production and cell viability of osteosarcoma cells. Overexpression of TUG1 induced cell viability, whereas the induction was attenuated by 2-DG. The aberrant expression of TUG1 markedly affected the expression of hexokinase-2 (HK2). Knockdown of HK2 weakened the effect of TUG1 overexpression on glycolysis in osteosarcoma cells. We concluded that glycolysis was involved in the effect of TUG1 on cell viability of osteosarcoma cells. HK2 might be an important molecule by which TUG1 affected the glycolysis.