Cleavage and secretion is not required for four-jointed function in Drosophila patterning
Cleavage and secretion is not required for four-jointed function in Drosophila patterning
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DOI：
10.1242/dev.00996
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发表时间：
2004-02-01
期刊：
影响因子：
4.6
通讯作者：
Strutt, D
中科院分区：
文献类型：
--
作者：
Strutt, H;Mundy, J;Strutt, D
在果蝇组织中，d四关节(，5)是近端生长和平面极性所必需的。它编码一个预测的11型跨膜蛋白，在其跨膜结构域中含有可能的信号肽，其C末端是分泌的。因此，FJ被认为是一种分泌的信号分子。我们发现Fj蛋白在眼睛和翅膀成像盘中呈梯度分布，并在体内和转基因细胞中主要定位于高尔基体。对构成分泌或锚定在高尔基体内的Fj形式进行了体内功能检测。我们发现Fj的切割和分泌不是活性所必需的，高尔基锚定的Fj比野生型有更高的活性。FI的表型与钙粘附素编码基因FAT(Ft)和Dachsous(Ds)突变引起的表型相似。我们发现FI与FT和DS在平面极性和近端图案上存在遗传上的相互作用。我们认为Fj可能作用于高尔基体，调节Ft和D的活性。
dfour-jointed (,5) is required for proximodistal growth and planar polarity in Drosophila tissues. It encodes a predicted type 11 transmembrane protein with putative signal peptidase sites in its transmembrane domain, and its C terminus is secreted. Fj has therefore been proposed to act as a secreted signalling molecule. We show that Fj protein has a graded distribution in eye and wing imaginal discs, and is largely localised to the Golgi in vivo and in transfected cells. Forms of Fj that are constitutively secreted or anchored in the Golgi were assayed for function in vivo. We find that cleavage and secretion of Fj is not necessary for activity, and that Golgi-anchored Fj has increased activity over wild type. fi has similar phenotypes to those caused by mutations in the cadherin-encoding genes fat (ft) and dachsous (ds). We show that fi interacts genetically with ft and ds in planar polarity and proximodistal patterning. We propose that Fj may act in the Golgi to regulate the activity of Ft and Ds.