Patterns of gene expression in different histotypes of epithelial ovarian cancer correlate with those in normal fallopian tube, endometrium, and colon
Patterns of gene expression in different histotypes of epithelial ovarian cancer correlate with those in normal fallopian tube, endometrium, and colon
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DOI：
10.1158/1078-0432.ccr-04-2509
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发表时间：
2005-09-01
影响因子：
11.5
通讯作者：
Lu, KH
中科院分区：
文献类型：
--
作者：
Marquez, RT;Baggerly, KA;Lu, KH
目的：卵巢上皮细胞[卵巢癌被认为是由覆盖在卵巢表面的扁平上皮细胞或包涵体囊泡形成的。在恶变过程中，出现了不同的组织类型，类似于正常输卵管、子宫内膜和肠道的上皮细胞。本研究比较浆液性、子宫内膜样癌、透明细胞癌和粘液性卵巢癌与其相似的正常组织中的基因表达。实验设计：检测了50例卵巢癌、5例正常卵巢上皮刷片池以及4例正常输卵管、5例子宫内膜和4例结肠标本的63,000个探针的表达。结果：卵巢浆液性肿瘤与正常卵巢组织相比，浆液性肿瘤的改变与正常输卵管组织无相关性(P=0.0042)。同样，粘液性癌与正常结肠粘膜相关(P=0.0003)，子宫内膜样癌和透明细胞组织类型与正常内膜的变化相关(P分别为0.0172和0.0002)。与正常卵巢上皮细胞相比，粘液性癌的基因表达变化最多。结论：分子水平的研究显示了卵巢癌不同组织的不同表达谱，并支持了长期以来的观点，即卵巢癌的组织类型逐渐类似于正常输卵管、子宫内膜和结肠上皮。粘液性卵巢癌的几个潜在分子标志物已经被确定。
Purpose: Epithelia[ ovarian cancers are thought to arise from flattened epithelial cells that cover the ovarian surface or that line inclusion cysts. During malignant transformation, different histotypes arise that resemble epithelial cells from normal fallopian tube, endometrium, and intestine. This study compares gene expression in serous, endometrioid, clear cell, and mucinous ovarian cancers with that in the normal tissues that they resemble.Experimental Design: Expression of 63,000 probe sets was measured in 50 ovarian cancers, in 5 pools of normal ovarian epithelial brushings, and in mucosal scrapings from 4 normal fallopian tube, 5 endometrium, and 4 colon specimens. Using rank-sum analysis, genes whose expressions best differentiated the ovarian cancer histotypes and normal ovarian epithelium were used to determine whether a correlation based on gene expression existed between ovarian cancer histotypes and the normal tissues they resemble.Results: When compared with normal ovarian epithelial brushings, alterations in serous tumors correlated with those in normal fallopian tube (P = 0.0042) but not in other normal tissues. Similarly, mucinous cancers correlated with those in normal colonic mucosa (P = 0.0003), and both endometrioid and clear cell histotypes correlated with changes in normal endometrium (P = 0.0172 and 0.0002, respectively). Mucinous cancers displayed the greatest number of alterations in gene expression when compared with normal ovarian epithelial cells.Conclusion: Studies at a molecular level show distinct expression profiles of different histologies of ovarian cancer and support the long-held belief that histotypes of ovarian cancers come to resemble normal fallopian tube, endometrial, and colonic epithelium. Several potential molecular markers for mucinous ovarian cancers have been identified.