The gene and messenger RNA for adenovirus polypeptide IX
The gene and messenger RNA for adenovirus polypeptide IX
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腺病毒多肽IX的基因和信使RNA
DOI：
10.1016/0092-8674(77)90274-4
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发表时间：
1977
期刊：
影响因子：
64.5
通讯作者：
M. Mathews
中科院分区：
文献类型：
--
作者：
U. Pettersson;M. Mathews
一个小RNA种类，从VA RNA不同，已被确定在HeLa细胞感染腺病毒2型。使用新的筛选程序纯化的RNA是聚腺苷酸化的，在9S处沉淀，估计长度为550个核苷酸。在无细胞翻译系统中，9S RNA指导病毒体多肽IX的合成，分子量为12，000道尔顿。其基因的位置已通过RNA与限制性内切酶切割产生的病毒DNA片段杂交确定：它跨越病毒基因组r链上的10.0位。这些结果出乎意料地将“晚期”蛋白质的基因放置在基因组的一个区域内，该区域在感染期间早期转录。
A small RNA species, distinct from the VA RNAs, has been identified in HeLa cells infected with adenovirus type 2. The RNA, which has been purified using a novel screening procedure, is polyadenylated, sediments at 9S and has an estimated length of 550 nucleotides. In a cell-free translation system, the 9S RNA directs the synthesis of virion polypeptide IX, molecular weight 12,000 daltons. The location of its gene has been established by hybridization of the RNA to fragments of viral DNA produced by cleavage with restriction endonucleases: it spans position 10.0 on the r strand of the viral genome. These results unexpectedly place the gene for a “late” protein within a region of the genome which is transcribed early during infection.