A GENETIC APPROACH TO PROMOTER RECOGNITION DURING TRANS INDUCTION OF VIRAL GENE-EXPRESSION
A GENETIC APPROACH TO PROMOTER RECOGNITION DURING TRANS INDUCTION OF VIRAL GENE-EXPRESSION
复制标题
DOI：
10.1126/science.3018926
复制
发表时间：
1986-10-03
期刊：
影响因子：
56.9
通讯作者：
MCKNIGHT, SL
中科院分区：
文献类型：
--
作者：
COEN, DM;WEINHEIMER, SP;MCKNIGHT, SL
哺乳动物细胞的病毒感染需要病毒基因表达的调节诱导。许多病毒基因的诱导，包括单纯疱疹病毒基因编码胸苷激酶（tk），依赖于病毒的调节蛋白，在反式作用。因为识别的tk启动子的细胞转录因子是很好的理解，其反式诱导的病毒调节蛋白可以作为一个有用的模型，真核基因表达的调节。因此，在单纯疱疹病毒的染色体tk启动子处引入了一组全面的突变，以直接分析启动子突变对tk转录的影响。在反式诱导条件下有效表达TK所需的启动子结构域对应于那些重要的细胞转录因子识别。因此，tk表达的反式诱导最初可能是由病毒调节蛋白与细胞转录因子的相互作用催化的。
Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors.