Differential in vivo regulation of steroid hormone receptor activation by Cdc37p.
Differential in vivo regulation of steroid hormone receptor activation by Cdc37p.
复制标题
Cdc37p 对类固醇激素受体激活的体内差异调节。
DOI：
10.1091/mbc.8.12.2501
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发表时间：
1997
影响因子：
3.3
通讯作者：
Caplan,AJ
中科院分区：
文献类型：
--
作者：
Fliss,AE;Fang,Y;Boschelli,F;Caplan,AJ
p60 v-src在酵母细胞中表达时，CDC 37基因对p60 v-src的活性是必需的。由于p60 v-src和类固醇激素受体的激活途径是相似的，本研究分析了雄激素受体和糖皮质激素受体在酵母细胞中表达的突变版本的theCDC 37基因的雄激素依赖性反式激活。在这个突变体中，雄激素受体的雄激素依赖性反式激活在允许和限制性温度下都是有缺陷的，尽管糖皮质激素受体的反式激活仅在限制性温度下有轻度缺陷。cdc 37 p似乎通过雄激素受体配体结合域发挥作用，但它不影响受体结合亲和力。Cdc 37 p调节类固醇激素受体的模型进行了讨论。
TheCDC37gene is essential for the activity of p60v-srcwhen expressed in yeast cells. Since the activation pathway for p60v-srcand steroid hormone receptors is similar, the present study analyzed the hormone-dependent transactivation by androgen receptors and glucocorticoid receptors in yeast cells expressing a mutant version of theCDC37gene. In this mutant, hormone-dependent transactivation by androgen receptors was defective at both permissive and restrictive temperatures, although transactivation by glucocorticoid receptors was mildly defective only at the restrictive temperature. Cdc37p appears to function via the androgen receptor ligand-binding domain, although it does not influence receptor hormone-binding affinity. Models for Cdc37p regulation of steroid hormone receptors are discussed.