A meiotic gene regulatory cascade driven by alternative fates for newly synthesized transcripts.
A meiotic gene regulatory cascade driven by alternative fates for newly synthesized transcripts.
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DOI：
10.1091/mbc.e10-05-0448
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Wise JA
中科院分区：
文献类型：
--
作者：
Cremona N;Potter K;Wise JA
分析32减数分裂基因从裂变酵母方面的新生转录，RNA加工/积累，和监视因子突变体的影响显示，绝大多数是“上”在增殖细胞和不到三分之一的减数分裂过程中显示出转录峰值，突出了RNA水平调控的重要贡献。为了确定裂殖酵母粟酒裂殖酵母从增殖到减数分裂分化过渡期间转录调节与RNA加工和周转的相对重要性，我们分析了RNA监视因子突变体对32个减数分裂基因表达的时间分布和影响。新生转录与稳态RNA积累的比较显示，这些基因中的绝大多数在最大RNA合成和峰值RNA积累之间显示出滞后。在减数分裂期间，总RNA水平平行于3′加工，其以多个时间上不同的波发生，在减数分裂诱导后3至6 h达到峰值。大多数早期基因和一个中间基因mei 4共享一种调节机制，其中一种专门的RNA监视因子靶向新合成的转录本进行破坏。Mei 4p是叉头转录因子家族的一员，反过来又调节下游基因的宿主。值得注意的是，在所调查的基因中，观察到不到三分之一的转录峰值，即使这些基因也显示出RNA水平调控的证据。总的来说，我们的研究结果使我们提出，由新合成的转录本的加工和稳定性的变化驱动的调节级联与众所周知的转录级联一起操作，因为裂变酵母细胞进入减数分裂。
Analyses of 32 meiotic genes from fission yeast with respect to nascent transcription, RNA processing/accumulation, and effects of surveillance factor mutants reveal that the vast majority are “on” in proliferating cells and less than one-third show a transcriptional peak during meiosis, highlighting the important contribution of RNA-level regulation. To determine the relative importance of transcriptional regulation versus RNA processing and turnover during the transition from proliferation to meiotic differentiation in the fission yeast Schizosaccharomyces pombe, we analyzed temporal profiles and effects of RNA surveillance factor mutants on expression of 32 meiotic genes. A comparison of nascent transcription with steady-state RNA accumulation reveals that the vast majority of these genes show a lag between maximal RNA synthesis and peak RNA accumulation. During meiosis, total RNA levels parallel 3′ processing, which occurs in multiple, temporally distinct waves that peak from 3 to 6 h after meiotic induction. Most early genes and one middle gene, mei4, share a regulatory mechanism in which a specialized RNA surveillance factor targets newly synthesized transcripts for destruction. Mei4p, a member of the forkhead transcription factor family, in turn regulates a host of downstream genes. Remarkably, a spike in transcription is observed for less than one-third of the genes surveyed, and even these show evidence of RNA-level regulation. In aggregate, our findings lead us to propose that a regulatory cascade driven by changes in processing and stability of newly synthesized transcripts operates alongside the well-known transcriptional cascade as fission yeast cells enter meiosis.