Evidence for luteal cell hyperplasia during pregnancy.
Evidence for luteal cell hyperplasia during pregnancy.
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怀孕期间黄体细胞增生的证据。
DOI：
10.1095/biolreprod44.3.511
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发表时间：
1991
影响因子：
3.6
通讯作者：
Hirshfield,AN
中科院分区：
文献类型：
--
作者：
Goldsmith,LT;Hirshfield,AN
大鼠妊娠期间黄体大小和重量的显著增加在很大程度上归因于黄体细胞肥大。一般认为，黄体细胞的增殖在黄体生长中不起作用，因为黄体细胞在体内不会发生分裂。最近的数据表明，情况可能并非如此。因此，为了确定大鼠在怀孕期间是否发生黄体细胞增殖，在怀孕第6天或第11天将充满~3H-胸腺嘧啶核苷的渗透性微泵植入定时妊娠大鼠体内。这些泵提供了每小时10微米的持续输注，持续时间长达7天。7天后(分别为第13天和第18天)处死大鼠，摘除卵巢，进行放射自显影。标记细胞具有黄体细胞的形态特征，在~3H-胸腺嘧啶核苷照射的卵巢放射自显影中清晰可见。这些细胞在妊娠6-13天的标记指数为6.0%，在妊娠11-18天的标记指数为1.2%。标记细胞的存在是否意味着黄体细胞的增殖，或者这些细胞是否来自另一种细胞类型，在形态上与黄体细胞相似，目前尚不清楚。然而，无论来源如何，这些数据都清楚地表明，黄体中的实质细胞数量在怀孕期间确实增加了。
The pronounced increase in luteal size and weight that occurs during rat pregnancy has been largely attributed to luteal cell hypertrophy. It is generally believed that hyperplasia does not play a role in luteal growth, since it is thought that luteal cell division in vivo does not occur. Recent data suggest that this may not be the case. Thus, to determine whether luteal cell hyperplasia occurs during rat pregnancy, osmotic minipumps filled with3H-thymidine were implanted in timed-pregnant rats on Day 6 or Day 11 of pregnancy. These pumps provided a continuous infusion of 10 µCi3H-thymidine per hour for up to 7 days. Seven days later (Day 13 and Day 18 respectively), rats were killed, and the ovaries were removed and prepared for autoradiography. Labeled cells, which have the morphological characteristics of luteal cells, were clearly observed in autoradiographs of ovaries exposed to3H-thymidine. The labeling index of these cells from ovaries exposed to3H-thymidine on Days 6–13 of pregnancy was 6.0% and from ovaries exposed to3H-thymidine on Days 11–18 of pregnancy was 1.2%. Whether the presence of labeled cells signifies proliferation of luteal cells or whether these cells are derived from another cell type that develops into cells morphologically similar to luteal cells is not known at present. However, regardless of origin, these data clearly demonstrate that the number of parenchymal cells in the corpus luteum does increase during pregnancy.