ZNF424 Induces Apoptosis and Inhibits Proliferation in Lung Carcinoma Cells.
ZNF424 Induces Apoptosis and Inhibits Proliferation in Lung Carcinoma Cells.
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ZNF424 诱导肺癌细胞凋亡并抑制增殖
DOI：
10.2174/1566524018666180705113642
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发表时间：
2018
影响因子：
2.5
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Liu W;Yuan W;Li X;Zhuang J;Mo X;Dai G;Wang Y;Chen J;Wan Y;Li Y;Zhu X;Chen Y;Luo S;Jiang Z;Shi Y;Chen F;Cao L;Ye X;Fan X;Zhu P;Zhang K;Wu X
背景资料：先前，我们发现含锌指的转录因子ZNF 424抑制p21转录，这与各种癌症广泛相关。然而，由于ZNF 424在肿瘤发生中的作用还没有得到表征，我们将ZNF 424表达与肺癌的肿瘤发生联系起来。结果如下：目前的免疫组化分析显示，与癌旁组织相比，60例肺癌组织中有43例的ZNF 424表达水平显著降低。流式细胞仪检测结果显示，ZNF 424过表达可诱导A549人肺癌细胞凋亡，且过表达ZNF 424可显著增加G1期细胞数，减少S期细胞数，提示ZNF 424可抑制A549人肺癌细胞增殖。Western Blot分析表明，ZNF 424的过表达降低了A549细胞中丝裂原活化蛋白激酶（MAPK）信号蛋白P-P38和P-ERK的蛋白表达水平。结论：这些是第一个将ZNF 424与肿瘤发生联系起来的数据，并证明了ZNF 424在肺癌中的抑制作用，表明ZNF 424表达作为肺肿瘤发生的诊断标志物的潜力。
Background: Previously, we showed that the Zinc finger-containing transcription factor ZNF424 inhibits p21 transcription, which has been widely associated with various cancers. However, because the roles of ZNF424 in tumorigenesis have not been characterized, we correlated ZNF424 expression with tumorigenesis in lung cancer. Results: The present immunohistochemical analyses show significantly lower ZNF424 expression levels in 43 of 60 lung cancer tissues compared with adjacent tissues. Moreover, flow cytometry assays indicated that overexpression of ZNF424 induces apoptosis in A549 human lung carcinoma cells, and overexpression of ZNF424 significantly increases numbers of G1 phase cells and decreases numbers of S phase cells, suggesting that ZNF424 inhibits proliferation. Western Blot analyses show that overexpression of ZNF424 decreases protein expression levels of the mitogen-activated protein kinase (MAPK) signaling proteins P-P38 and P-ERK in A549 cells. Conclusion: These are the first data to associate ZNF424 with tumorigenesis and demonstrate an inhibitory role in lung cancer, indicating the potential of ZNF424 expression as a diagnostic marker of lung tumorigenesis.