Separation and detection of individual Aβ aggregates by capillary electrophoresis with laser-induced fluorescence detection
Separation and detection of individual Aβ aggregates by capillary electrophoresis with laser-induced fluorescence detection
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DOI：
10.1016/j.ab.2012.03.006
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发表时间：
2012-06-15
影响因子：
2.9
通讯作者：
Gilman, S. Douglass
中科院分区：
文献类型：
--
作者：
Picou, Ryan A.;Schrum, David P.;Gilman, S. Douglass
演示了通过毛细管电泳结合激光诱导荧光检测 (CE-LIF) 来分离和检测单个淀粉样蛋白 (Aβ) 聚集体。使用 A beta (1-40) 或 A beta (1-42) 肽制备样品，并通过 CE、紫外线 (UV) 吸光度检测和透射电子显微镜 (TEM) 进行表征。在电泳缓冲液中使用硫代黄素 T (ThT)，含有聚集体的样品的电泳（5.0 秒进样）产生多达数百个窄带（
The separation and detection of individual amyloid beta (A beta) aggregates by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was demonstrated. Samples were prepared with either A beta (1-40) or A beta (1-42) peptides and were characterized by CE with ultraviolet (UV) absorbance detection and transmission electron microscopy (TEM). Using thioflavin T (ThT) in the electrophoresis buffer, electrophoresis of aggregate-containing samples (5.0-s injection) produced up to several hundred narrow (