INDUCEMENT OF CYTOPATHIC CHANGES AND PLAQUE-FORMATION BY PORCINE HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS
INDUCEMENT OF CYTOPATHIC CHANGES AND PLAQUE-FORMATION BY PORCINE HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS
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DOI：
10.1016/0378-1135(83)90001-9
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发表时间：
1983-01-01
影响因子：
3.3
通讯作者：
MATUMOTO, M
中科院分区：
文献类型：
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作者：
SATO, K;INABA, Y;MATUMOTO, M
埃斯克[猪胚肾]细胞被证明是繁殖猪血凝性脑脊髓炎病毒67 N株的良好培养基，尽管没有观察到细胞病变效应。当在培养基中掺入非细胞毒性量的二乙基氨基乙基-葡聚糖（DEAE-葡聚糖）时，病毒在埃斯克细胞中诱导容易识别的细胞病变效应。基于这一发现，开发了一种灵敏、实用的病毒检测方法。当DEAE-葡聚糖掺入琼脂覆盖培养基中时，67 N病毒在埃斯克细胞单层中形成噬斑。抗病毒血清能特异性地抑制细胞病变和空斑形成。中和试验是在这些发现的基础上进行的。对猪血清样本进行的中和和血凝抑制试验表明，血凝性脑脊髓炎病毒或抗原相关病毒在日本猪中广泛传播。
ESK [swine embryo kidney] cells were shown to be a good medium for propagating the 67N strain of porcine hemagglutinating encephalomyelitis virus, although no cytopathic effect was observed. The virus induced a readily recognizable cytopathic effect in ESK cells, when a non-cytotoxic amount of diethylaminoethyl-dextran (DEAE-dextran) was incorporated in the culture medium. Based on this finding, a sensitive, practical assay method for the virus was developed. When DEAE-dextran was incorporated in the agar overlay medium, 67N virus formed plaques in ESK cell monolayers. The cytopathic effect and the plaque formation were specifically inhibited by antisera against the virue. Neutralization tests were develped on the basis of these findings. Neutralization and hemagglutination-inhibition tests on swine serum samples indicated a wide dissemination of hemagglutinating encephalomyelitis virus or antigenically-related viruses in Japanese pigs.