Polymorphism in the acetylcholinesterase gene of the housefly, Musca domestica L. (Diptera: Muscidae)
Polymorphism in the acetylcholinesterase gene of the housefly, Musca domestica L. (Diptera: Muscidae)
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DOI：
10.1303/aez.2001.377
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发表时间：
2001-08-01
影响因子：
1.3
通讯作者：
Kono, Y
中科院分区：
文献类型：
--
作者：
Kozaki, T;Shono, T;Kono, Y
对家蝇乙酰胆碱酯酶（AChE）基因的cDNA片段进行了比较。虽然在预测的肽中在Asp（83）和Arg（648）之间有4个氨基酸取代，这在昆虫中是很保守的，但在菌株之间进行了以下对比。抗性菌株LPR、YBOL和YPRN分别含有Ala（342）和Tyr（407），而YBOL只有瓦尔（342）。此外，Leu（260）仅在手性有机磷抗性菌株YBOL和YPRN中发现。这些结果表明，单核苷酸多态性的模式，导致氨基酸取代，可以是一个实用的工具，用于表征杀虫剂抗性的家蝇。
The cDNA fragments of a 2kb gene, encoding the housefly acetylcholinesterase (AChE) were compared among 6 strains. While there were 4 substitutions of amino acids in the predicted peptides between Asp(83) and Arg(648), which is well conserved in insects, the following contrasts were made among the strains. The insecticide susceptible strains, aabys and SRS had Gly(342) and Phe(407), and the resistant strains, LPR, YBOL and YPRN had Ala(342) and Tyr(407), with Val(342) unique to YBOL. In addition, Leu(260) was found only in the chiral organophosphate resistant strains, YBOL and YPRN. These results showed that the patterns of single nucleotide polymorphisms, which resulted in amino acid substitutions, can be a practical tool for characterization of the insecticide resistance of houseflies.