Bactericidal Permeability-Increasing Proteins Shape Host-Microbe Interactions.
Bactericidal Permeability-Increasing Proteins Shape Host-Microbe Interactions.
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DOI：
10.1128/mbio.00040-17
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发表时间：
2017-04-04
期刊：
影响因子：
6.4
通讯作者：
McFall-Ngai MJ
中科院分区：
文献类型：
--
作者：
Chen F;Krasity BC;Peyer SM;Koehler S;Ruby EG;Zhang X;McFall-Ngai MJ
我们对鱿鱼 Euprymna scolopes 的杀菌渗透性增加蛋白 (BPI) EsBPI2 和 EsBPI4 进行了表征。它们具有其他动物 BPI 的典型分子特征，彼此密切相关，并且在系统发育上属于无脊椎动物 BPI。纯化的 EsBPI 对乌贼的共生体费氏弧菌（Vibrio fischeri）具有抗菌活性，该弧菌定植于光器官隐窝上皮细胞。热处理和与特异性抗体共孵育可消除两种蛋白质的活性。在类似于共生起始期间的酸性条件下进行预处理，使得费氏弧菌对蛋白质的抗菌活性具有更强的抵抗力。免疫细胞化学将 EsBPI 定位于与周围海水相互作用的共生器官和其他上皮表面。这些蛋白质在细胞内的分布不同。此外，EsBPI4 仅限于上皮细胞，而 EsBPI2 也存在于血液和介导孵化的短暂幼体器官中。这些数据提供的证据表明，这些 BPI 在 E. scolopes 生命的早期发挥着不同的防御作用，调节与共生体的相互作用。这项研究描述了杀菌渗透性增加蛋白 (BPI) 的新功能，BPI 是脂多糖结合蛋白 (LBP)/BPI 蛋白家族的成员。这些数据证明这些蛋白质在共生细菌的调节中发挥双重作用。在鱿鱼-弧菌模型中，这些蛋白质既控制光器官组织中的共生体群体，其中共生体细胞以密集的单一培养物存在，同时抑制共生体定植于动物的其他上皮表面。
We characterized bactericidal permeability-increasing proteins (BPIs) of the squid Euprymna scolopes, EsBPI2 and EsBPI4. They have molecular characteristics typical of other animal BPIs, are closely related to one another, and nest phylogenetically among invertebrate BPIs. Purified EsBPIs had antimicrobial activity against the squid’s symbiont, Vibrio fischeri, which colonizes light organ crypt epithelia. Activity of both proteins was abrogated by heat treatment and coincubation with specific antibodies. Pretreatment under acidic conditions similar to those during symbiosis initiation rendered V. fischeri more resistant to the antimicrobial activity of the proteins. Immunocytochemistry localized EsBPIs to the symbiotic organ and other epithelial surfaces interacting with ambient seawater. The proteins differed in intracellular distribution. Further, whereas EsBPI4 was restricted to epithelia, EsBPI2 also occurred in blood and in a transient juvenile organ that mediates hatching. The data provide evidence that these BPIs play different defensive roles early in the life of E. scolopes, modulating interactions with the symbiont. This study describes new functions for bactericidal permeability-increasing proteins (BPIs), members of the lipopolysaccharide-binding protein (LBP)/BPI protein family. The data provide evidence that these proteins play a dual role in the modulation of symbiotic bacteria. In the squid-vibrio model, these proteins both control the symbiont populations in the light organ tissues where symbiont cells occur in dense monoculture and, concomitantly, inhibit the symbiont from colonizing other epithelial surfaces of the animal.