GABA promotes elastin synthesis and elastin fiber formation in normal human dermal fibroblasts (HDFs)
GABA promotes elastin synthesis and elastin fiber formation in normal human dermal fibroblasts (HDFs)
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DOI：
10.1080/09168451.2017.1290518
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发表时间：
2017-02
期刊：
影响因子：
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通讯作者：
Eriko Uehara;Hideki Hokazono;M. Hida;Takako Sasaki;H. Yoshioka;Noritaka Matsuo
中科院分区：
文献类型：
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作者：
Eriko Uehara;Hideki Hokazono;M. Hida;Takako Sasaki;H. Yoshioka;Noritaka Matsuo
氨基丁酸(γ-氨基丁酸)作为一种功能食品成分具有多种生理效应。我们之前曾报道，GABA上调人真皮成纤维细胞(HDF)I型胶原的表达，口服GABA显著增加皮肤弹性。然而，监管机制的细节仍不得而知。在这项研究中，我们进一步研究了GABA对HDFS中弹性蛋白合成和弹性蛋白纤维形成的影响。实时荧光定量聚合酶链式反应显示，GABA显著增加原弹性蛋白转录本的表达，并呈剂量依赖关系。此外，纤维蛋白-1、纤维蛋白-2和纤维蛋白-5/Dance的表达也显著增加，而赖氨酰氧化酶和潜伏性转化因子-β结合蛋白4的表达无明显变化。最后，免疫组织化学分析证实，GABA处理显著增加了HDFS中弹性纤维的形成。综上所述，我们的结果表明，GABA通过上调弹性蛋白合成和弹性蛋白纤维形成来改善HDFS患者的皮肤弹性。
The multiple physiological effects of γ-aminobutyric acid (GABA) as a functional food component have been recently reported. We previously reported that GABA upregulated the expression of type I collagen in human dermal fibroblasts (HDFs), and that oral administration of GABA significantly increased skin elasticity. However, details of the regulatory mechanism still remain unknown. In this study, we further examined the effects of GABA on elastin synthesis and elastin fiber formation in HDFs. Real-time PCR indicated that GABA significantly increased the expression of tropoelastin transcript in a dose-dependent manner. Additionally, the expression of fibrillin-1, fibrillin-2, and fibulin-5/DANCE, but not lysyl oxidase and latent transforming factor-β-binding protein 4, were also significantly increased in HDFs. Finally, immunohistochemical analysis confirmed that treatment with GABA dramatically increased the formation of elastic fibers in HDFs. Taken together, our results showed that GABA improves skin elasticity in HDFs by upregulating elastin synthesis and elastin fiber formation.