Enhancement of bone formation by BMP-7 transduced MSCs on biomimetic nano-hydroxyapatite/polyamide composite scaffolds in repair of mandibular defects
Enhancement of bone formation by BMP-7 transduced MSCs on biomimetic nano-hydroxyapatite/polyamide composite scaffolds in repair of mandibular defects
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DOI：
10.1002/jbm.a.32926
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发表时间：
2010-12-15
影响因子：
4.9
通讯作者：
Hu, Jing
中科院分区：
文献类型：
--
作者：
Li, Jihua;Li, Yubao;Hu, Jing
本研究旨在评价骨形态发生蛋白-7(BMP-7)转导的骨髓间充质干细胞在纳米羟基磷灰石/聚酰胺(n-HA/PA)复合支架上促进骨形成的作用，为骨组织工程修复下颌骨缺损提供实验依据。制备N-HA/PA支架，分离、扩增兔MSCs，体外感染腺病毒介导的BMP-7。将MSCs-BMP-7和MSCs种植在多孔支架上。将支架/MSCs-BMP-7支架和支架/MSCs支架分别植入兔下颌骨缺损区，分别为实验A组(n=18)和B组(n=18)，单纯支架植入作为对照(C组，n=18)。分别于移植后4周、8周和16周处死6只动物。取下下颌骨进行放射学、生物力学测试、组织学和组织形态计量学分析。A组动物在移植后4周和8周时骨形成和矿化早于B组，B组动物在移植后4周和8周高于C组，但在移植后16周，三组动物之间没有差异。本研究结果提示，BMP-7转导的MSCs-n-HA/PA复合材料可明显促进种植体的早期成骨。BMP-7介导的以骨髓间充质干细胞为种子细胞的体外基因转移，复合多孔n-HA/PA作为骨组织工程支架材料，可能是修复下颌骨缺损的一种替代或补充方法。(C)2010年威利期刊公司生物材料资源A部分：95A：973-981,2010年。
This study was to evaluate enhanced bone formation by bone morphogenetic protein-7 (BMP-7) transduced MSCs on nano-hydroxyapatite/polyamide (n-HA/PA) composite scaffolds for bone tissue engineering in repair of mandibular defect. n-HA/PA scaffolds were prepared and rabbit MSCs were separated and expanded; and then infected with adenoviral-mediated BMP-7 in vitro. The MSCs-BMP-7 and MSCs were seeded on the porous scaffolds. Scaffold/MSCs-BMP-7 constructs and scaffold/MSCs constructs were implanted in the defects of rabbits' mandible as the experimental groups A (n = 18) and groups B (n = 18), respectively, the pure scaffolds were implanted as controls (group C, n = 18). Six animals were sacrificed at 4-, 8-, and 16-week postimplantation, respectively. Their mandibles were removed and processed for radiographic, biomechanical tests, histological, and histomorphometric analysis. Group A animals showed greater bone formation and earlier mineralization than group B at 4- and 8-week postimplantation and similarly group B more than group C. However, no difference was found among three groups at 16-week postimplantation. The results of this study suggest that BMP-7 transduced MSCs-n-HA/PA composite could significantly accelerate bone formation in the implant at early stage. BMP-7 mediated ex vivo gene transfer based on MSCs as seed cells, combined with porous n-HA/PA as scaffolds for bone tissue engineering might be an alternative or supplemental approach to repair the mandibular defects. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 973-981, 2010.