Effects of light, macronutrients, and sucrose on germination and development of the endangered fern Adiantum reniforme var. sinense (Adiantaceae)
Effects of light, macronutrients, and sucrose on germination and development of the endangered fern Adiantum reniforme var. sinense (Adiantaceae)
复制标题
光、常量营养素和蔗糖对濒危蕨类植物铁线蕨 (Adiantum reniforme var.) 发芽和发育的影响。
DOI：
10.1016/j.scienta.2010.03.004
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发表时间：
2010-06-28
影响因子：
4.3
通讯作者：
Chen Long-Qing
中科院分区：
文献类型：
--
作者：
Wu Hua;Liu Xiu-Qun;Chen Long-Qing
研究了光照、培养基中大量营养元素浓度（MS，1962）和蔗糖对濒危蕨类荷叶铁线蕨（Adiantum reniforme var.）孢子萌发和配子体发育的影响。sinense进行了调查。光的存在被认为是必不可少的孢子萌发和配子体生长。1/4 MS +15 g/l蔗糖是孢子萌发和配子体发育的最适培养基。MS +30 g/l蔗糖是配子体进一步发育的最佳培养基。增加培养基中蔗糖的量（45-60 g/l）延迟配子体的生长和发育。此外，在粘土和泥炭（v/v = 1：2）组成的培养基中孢子体的形成和配子体的早期生长均高于纯河沙组成的培养基。这些结果表明，黑曲霉孢子萌发和配子体早期发育对营养物质的需求是有限的。肾形变种中华根瘤菌相对较低，但随着配子体的进一步发育、孢子体的形成和生长而增加。(C)2010 Elsevier B. V.保留所有权利。
Effects of light, macronutrients strength of Murashige and Skoog (MS) (1962), and sucrose in the culture medium on spore germination and gametophyte development of the endangered fern Adiantum reniforme var. sinense were investigated. The presence of light was found to be essential for both spore germination and gametophyte growth. Moreover, a medium consisting of 1/4 MS with 15 g/l sucrose was optimal for spore germination and early gametophyte development; whereas. MS medium with 30 g/l sucrose was optimal for further gametophyte development. Increasing amounts of sucrose (45-60 g/l) in the medium delayed gametophyte growth and development. Additionally, sporophyte formation and early growth of gametophytes in a medium consisting of clay and peat (v/v = 1:2) was higher than those in a medium consisting of pure river sand. These findings indicated that requirements for nutrients for spore germination and early gametophyte development of A. reniforme var. sinese were relatively low, but these increased with further gametophyte development, formation and growth of sporophytes. (C) 2010 Elsevier B.V. All rights reserved.