Isolation and characterization of a gene for assimilatory sulfite reductase from Arabidopsis thaliana
Isolation and characterization of a gene for assimilatory sulfite reductase from Arabidopsis thaliana
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DOI：
10.1016/s0378-1119(98)00155-3
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发表时间：
1998-05-28
期刊：
影响因子：
3.5
通讯作者：
Hell, R
中科院分区：
文献类型：
--
作者：
Bork, C;Schwenn, JD;Hell, R
亚硫酸盐还原酶（SIR）是高等植物硫酸盐同化的关键酶。分离并鉴定了拟南芥sir基因的基因组DNA序列，包括调控区和结构区。对编码SIR的6 kb片段进行测序，发现编码区长2891 bp，由8个外显子和7个内含子组成，长度在83 - 139 bp之间。转录起始点确定为翻译起始位点上游272 bp处。Southern分析表明，sir基因的一个单一位点在叶和根中产生2.4（kb）的mRNA。通过gusA报告基因在转基因A. thaliana植物中，并显示在根和叶中提供正确的表达。(C)1998 Elsevier Science B. V.保留所有权利。
Sulfite reductase (SIR) represents a key enzyme in sulfate assimilation in higher plants. The genomic DNA sequence of the sir gene from Arabidopsis thaliana including regulatory and structural regions was isolated and characterized. The sequence of a 6 kb fragment encoding SIR revealed a coding region of 2891 basepairs (bp) that consists of eight exons separated by seven introns between 83 and 139 bp in length. The transcription start point was determined 272 bp upstream of the translation start site. Southern analysis indicates a single locus for the sir gene that gives rise to a 2.4 (kb) mRNA in leaves and in roots. The promoter region was verified by functional expression of the gusA reporter gene in transgenic A. thaliana plants and was shown to provide correct expression in root and leaf. (C) 1998 Elsevier Science B.V. All rights reserved.