Phylogenetic analysis of alphaviruses in the Venezuelan equine encephalitis complex and identification of the source of epizootic viruses.
Phylogenetic analysis of alphaviruses in the Venezuelan equine encephalitis complex and identification of the source of epizootic viruses.
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委内瑞拉马脑炎复合体中甲病毒的系统发育分析和流行病毒来源的鉴定。
DOI：
10.1016/0042-6822(92)90190-z
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发表时间：
1992
期刊：
影响因子：
3.7
通讯作者：
Rico-Hesse,R
中科院分区：
文献类型：
--
作者：
Weaver,SC;Bellew,LA;Rico-Hesse,R
我们使用代表性毒株nsP 4、El和3′非翻译基因组区域有限部分的RNA核苷酸序列的系统发育分析，研究了委内瑞拉马脑炎（VEE）复合体中甲病毒的进化。VEE复合体构成了病毒的单系群（来自共同祖先）;一些血清学VEE变种，如亚型III形成了单系群，而亚型I则没有。II型沼泽地和品种ID地方流行病毒形成了一个单系群，其中还包括所有的流行品种IAB和IC VEE分离株。大沼泽地病毒从这个ID谱系（殖民北美）约。100-150年前，随后IAB和IC流行病毒品种出现分歧。品种IAB病毒可能在本世纪早期从品种ID谱系中出现一次，而品种IC病毒至少进化了两次。这些结果将动物流行性VEE病毒的来源确定为在南美洲北方和巴拿马发生的品种ID地方病病毒谱系。即使品种IAB和IC病毒已经灭绝，最近，来自地方病谱系的动物流行病毒的多次出现表明，其他动物流行VEE病毒可能在未来再次进化。II型大沼泽地病毒与品种ID谱系的密切遗传关系也意味着在佛罗里达可能出现马毒性VEE病毒。
We studied the evolution of alphaviruses in the Venezuelan equine encephalitis (VEE) complex using phylogenetic analysis of RNA nucleotide sequences from limited portions of the nsP4, El, and 3′ untranslated genome regions of representative strains. The VEE complex constituted a monophyletic group of viruses (descended from a common ancestor); some serologic VEE varieties such as subtype III formed monophyletic groups while subtype I did not. Subtype II Everglades and variety ID enzootic viruses formed a monophyletic group which also included all epizootic variety IAB and IC VEE isolates. Everglades virus diverged from this ID lineage (colonized North America) ca. 100–150 years ago, followed by divergence of variety IAB and IC epizootic viruses. Variety IAB viruses probably emerged from the variety ID lineage once during the early part of this century, while variety IC viruses evolved at least two times. These results identify the source of epizootic VEE viruses as the variety ID enzootic virus lineage which occurs in northern South America and Panama. Even if variety IAB and IC viruses are extinct, recent, multiple emergences of epizootic viruses from an enzootic lineage suggests that other epizootic VEE viruses may evolve again in the future. The close genetic relationship of subtype II Everglades virus to the variety ID lineage also implies the potential for emergence of equine-virulent VEE viruses in Florida.