Preferential binding of E. coli with type 1 fimbria to D-mannobiose with the Manα1→2Man structure
Preferential binding of E. coli with type 1 fimbria to D-mannobiose with the Manα1→2Man structure
复制标题
具有 1 型伞毛的大肠杆菌优先结合具有 Manα1→2Man 结构的 D-甘露二糖
DOI：
10.1080/09168451.2015.1075863
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Ajisaka;K.;Yuki;K.;Sato;K.;Ishii;N.;Matsuo;I.;Kuji;R.;Miyazaki;T.;and Furukawa;K.
Manα1→ 2 Man、Manα1→ 3 Man、Manα1→ 4 Man和Manα1→ 6 Man转化为糖胺衍生物。然后，将它们与单苄基琥珀酸混合以获得它们的酰胺衍生物。通过氢化除去苄基后，在水溶性碳二亚胺的存在下，将琥珀酰胺衍生物与BlotGlyco™珠上的肼基偶联。将d-甘露二糖连接的珠与荧光标记的具有1型菌毛的大肠杆菌一起孵育，并计数与珠相关的荧光点的数量，以确定结合偏好的d-甘露二糖异构体。结果表明，细菌与Manα1→ 2 Man 1 →beads、Manα1→ 3 Man 1 →beads、Manα1→ 4 Man 1 →beads和Manα1→ 6 Man 1 →beads的结合强度依次为强。在0.1 M甲基α-d-吡喃甘露糖苷存在下，大多数细菌不能与这些珠子结合。这些结果表明，E. 1型菌毛的大肠杆菌与所有类型的甘露二糖异构体结合，但优先与Manα1→ 2 Man二糖结合。
Manα1→2Man, Manα1→3Man, Manα1→4Man, and Manα1→6Man were converted to the glycosylamine derivatives. Then, they were mixed with monobenzyl succinic acid to obtain their amide derivatives. After removing the benzyl group by hydrogenation, the succinylamide derivatives were coupled with the hydrazino groups on BlotGlyco™ beads in the presence of water-soluble carbodiimide.d-Mannobiose-linked beads were incubated with fluorescence-labeledEscherichia coliwith type 1 fimbria, and the number of the fluorescent dots associated with the beads was counted in order to determine the binding preference amongd-mannobiose isomers. The results showed that the bacteria bind strongly to Manα1→2Man1→beads, Manα1→3Man1→beads, Manα1→4Man1→beads, and Manα1→6Man1→beads, in order. In the presence of 0.1 M methyl α-d-mannopyranoside, most of the bacteria failed to bind to these beads. These results indicate thatE. coliwith type 1 fimbria binds to all types ofd-mannobiose isomers but preferentially to Manα1→2Man disaccharide.