Mucinous bronchioloalveolar carcinomas display a specific pattern of mucin gene expression among primary lung adenocarcinomas
Mucinous bronchioloalveolar carcinomas display a specific pattern of mucin gene expression among primary lung adenocarcinomas
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DOI：
10.1053/hupa.2001.22752
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发表时间：
2001-03-01
期刊：
影响因子：
3.3
通讯作者：
Porchet, N
中科院分区：
文献类型：
--
作者：
Copin, MC;Buisine, MP;Porchet, N
肺腺癌在临床和组织学上是异质性的。粘蛋白基因的表达作为原发性肺腺癌腺细胞分化的分子标志物进行了分析。应用原位杂交技术检测34例肺腺癌组织中MUC 1 -4、MUC 5AC、MUC 5 B、MUC 6 -7基因的表达，免疫组化法检测MUC 5AC和MUC 5 B核粘蛋白的表达。粘液性细支气管肺泡癌（BAG）具有与其他类型肺腺癌不同的粘蛋白基因表达的均一模式，包括分泌型粘蛋白（MUC 5AC、MUC 5 B和MUC 6）和膜结合型粘蛋白（MUC 1、MUC 3和MUC 4）。非BAG腺癌和粘液性BAC分别异常表达粘蛋白基因MUC 3、MUC 3和MUC 6，这在正常胎儿和成人肺中是检测不到的。我们的研究结果表明，粘蛋白基因的表达在粘液型BAC的特殊表型和呼吸和非呼吸性粘蛋白在其他类型的异质性表达。这一发现支持了具有多细胞分化潜能的共同祖细胞的理论。从实用的角度来看，MUC 3和MUC 6的异常表达可以作为粘液型细支气管肺泡癌的诊断标志物。《人文哲学》32：274-281。Copyright（C）2001 by W.B.桑德斯公司
Lung adenocarcinomas are heterogeneous clinically and histologically. Expression of the mucin genes was analyzed as a molecular marker of glandular cytodifferentiation in primary lung adenocarcinomas. Expression was correlated with histopathologic subtypes of World Health Organization classification with the aim of investigating the histogenesis of primary lung adenocarcinomas, Thirty-four primary lung adenocarcinomas were examined by in situ hybridization for mucin gene expression (MUC1-4, MUC5AC,MUC5B, MUC6-7) and by immunohistochemistry for MUC5AC and MUC5B apomucin expression. Mucinous bronchioloalveolar carcinoma (BAG) had a homogeneous pattern of mucin gene expression different from those of other types of lung adenocarcinoma, involving secreted mucins (MUC5AC, MUC5B, and MUC6) and membrane-bound mucins (MUC1, MUC3, and MUC4). Non-BAG adenocarcinoma and mucinous BAC aberrantly expressed mucin genes MUC3, and MUC3 and MUC6, respectively, which are undetectable in normal fetal and adult lung. Our results show the particular phenotype of mucin gene expression in mucinous type of BACs and the heterogeneous expression of respiratory and nonrespiratory mucins in the other types. This finding supports the theory of a common progenitor cell with the potential of multicellular differentiation. From a practical point of view, the aberrant expression of MUC3 and MUC6 could serve as a diagnostic marker in the management of the mucinous type of bronchioloalveolar carcinomas. HUM PATHOL 32:274-281. Copyright (C) 2001 by W.B. Saunders Company.