Influence of the diet components on the symbiotic microorganisms community in hindgut of Coptotermes formosanus Shiraki
Influence of the diet components on the symbiotic microorganisms community in hindgut of Coptotermes formosanus Shiraki
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DOI：
10.1007/s00253-005-0215-3
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发表时间：
2006-03
影响因子：
5
通讯作者：
Hideo Tanaka;H. Aoyagi;Syunsuke Shina;Yuri Dodo;T. Yoshimura;Ryosuke Nakamura;H. Uchiyama
中科院分区：
文献类型：
--
作者：
Hideo Tanaka;H. Aoyagi;Syunsuke Shina;Yuri Dodo;T. Yoshimura;Ryosuke Nakamura;H. Uchiyama
本文报道了台湾乳白蚁（Coptotermes formosanus）人工饲料的研制。用日本红松木粉、纤维素、纤维二糖或葡萄糖饲喂台湾竹荪30天。研究了日粮中不同碳源对肠道共生微生物群落结构和功能的影响以及对C.研究了福尔摩沙。结果表明，在该虫后肠中发现了3种共生原虫，即草拟毛管虫（Pseudotrichonymphagrassi）、哈氏全鞭毛虫（Holomastigotoides hartmanni）和莱迪螺旋毛管虫（Spirotrichonymphaleidyi）。以含高分子量碳源的饲料饲养的台湾对虾。而在低分子量碳水化合物的人工饲料中，P. grassiandH.哈特曼尼消失了，只有少数人。莱迪还活着。这表明，bothP。grassiandH.哈特曼氏菌在高分子量碳水化合物的消化和利用中起重要作用。对白蚁后肠细菌群落的变性梯度凝胶电泳分析表明，饲喂高分子量碳源饲料的白蚁后肠细菌群落与饲喂低分子量碳源饲料的白蚁后肠细菌群落的相似性仅为40%左右。由此可见，日粮的变化会导致肠道微生物群落的变化，进而影响纤维素酶活性。福尔摩沙人。
Artificial diet was developed for rearing of lower termites (workers)Coptotermes formosanus.C. formosanuswas fed with either wood powder of Japanese red pine, cellulose, cellobiose, or glucose for 30 days. The effect of carbon sources in the diet on the structure and function of the symbiotic intestinal microbial community and on the physiological activity ofC. formosanuswas studied. Three symbiont protozoa,Pseudotrichonympha grassi,Holomastigotoides hartmanni, andSpirotrichonympha leidyi, were found in the hindgut ofC. formosanusthat fed on the diets containing carbon sources with high molecular weight (MW). However, when artificial diets containing carbohydrate with low MW were used, bothP. grassiandH. hartmannidisappeared, and only fewS. leidyiwere alive. This suggested that bothP. grassiandH. hartmanniplay important roles in the digestion and utilization of carbohydrate with high MW. The denaturing gradient gel electrophoresis analysis of bacterial community in the hindgut of termites showed that the similarity between intestinal bacteria community in termites fed with diets containing high-MW carbon sources and those with low MW was only about 40%. It was apparent that changes in diets resulted to changes in intestinal microbial community, and this in turn affected cellulase activity inC. formosanus.