Control of cell fate by the formation of an architecturally complex bacterial community
Control of cell fate by the formation of an architecturally complex bacterial community
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DOI：
10.1101/gad.1645008
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发表时间：
2008-04-01
影响因子：
10.5
通讯作者：
Kolter, Roberto
中科院分区：
文献类型：
--
作者：
Vlamakis, Hera;Aguilar, Claudio;Kolter, Roberto
细菌形成了被称为生物膜的结构复杂的群落，其中的细胞由细胞外基质结合在一起。生物膜具有多种细胞类型，有研究认为，生物膜内单个细胞遵循不同的发育路径，导致种群的异质性。在这里，我们展示了芽胞形成细菌枯草芽孢杆菌生物膜内的细胞分化，并提出了生物膜形成支配分化的证据。我们表明，可移动的、产生基质的和产孢子的细胞定位于生物膜内的不同区域，并且每种细胞类型的定位和百分比在群落的整个发展过程中是动态的。重要的是，不产生细胞外基质的突变体形成缺乏产孢量的非结构化生物膜。我们认为，产孢子是生物膜形成的最终特征，而孢子形成与结构复杂的细胞群落的形成是耦合的。
Bacteria form architecturally complex communities known as biofilms in which cells are held together by an extracellular matrix. Biofilms harbor multiple cell types, and it has been proposed that within biofilms individual cells follow different developmental pathways, resulting in heterogeneous populations. Here we demonstrate cellular differentiation within biofilms of the spore-forming bacterium Bacillus subtilis, and present evidence that formation of the biofilm governs differentiation. We show that motile, matrix-producing, and sporulating cells localize to distinct regions within the biofilm, and that the localization and percentage of each cell type is dynamic throughout development of the community. Importantly, mutants that do not produce extracellular matrix form unstructured biofilms that are deficient in sporulation. We propose that sporulation is a culminating feature of biofilm formation, and that spore formation is coupled to the formation of an architecturally complex community of cells.