Kinetic model of double-stranded RNA formation during long RNA replication by Qβreplicase
Kinetic model of double-stranded RNA formation during long RNA replication by Qβreplicase
复制标题
Qβ复制酶复制长RNA过程中双链RNA形成的动力学模型
DOI：
10.1016/j.febslet.2013.06.033
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发表时间：
2013
期刊：
影响因子：
3.5
通讯作者：
T
中科院分区：
文献类型：
--
作者：
Usui;K.;Ichihashi;N.;Kazuta;Y.;Matsuura;T.;Yomo;T
Qβ复制酶是一种依赖于RNA的RNA聚合酶，以单链RNA为模板合成互补RNA。新合成的RNA与模板RNA杂交形成不可复制的双链RNA（dsRNA）阻碍了Qβ复制酶的广泛应用。在此，我们建立了一个由两条dsRNA形成反应途径组成的Qβ RNA复制动力学模型，定量地解释了三种模板RNA的dsRNA形成动力学。我们还发现Qβ噬菌体基因组RNA序列的一部分，包括中央发夹环，显着降低dsRNA的形成速率。
Qβ replicase is an RNA-dependent RNA polymerase, which synthesizes the complementary RNA using a single-stranded RNA as a template. The formation of non-replicable double-stranded RNA (dsRNA) by hybridization between newly synthesized RNA and the template RNA hinders the broader application of Qβ replicase. Here, we developed a kinetic model of Qβ RNA replication consisting of two reaction pathways of dsRNA formation, which quantitatively explains the dynamics of dsRNA formation of three template RNAs. We also found that part of the Qβ phage genomic RNA sequence including the central hairpin loop significantly decreases the rate of dsRNA formation.