The tissue-engineered vascular graft using bone marrow without culture
The tissue-engineered vascular graft using bone marrow without culture
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DOI：
10.1016/j.jtcvs.2004.10.030
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发表时间：
2005-05-01
影响因子：
6
通讯作者：
Kurosawa, H
中科院分区：
文献类型：
--
作者：
Hibino, N;Shin'oka, T;Kurosawa, H
目的：为克服现有血管移植物的不足，将组织工程方法应用于心血管领域。我们以前曾报道过使用血管混合细胞建立组织工程化血管移植物。然而，细胞的采集和培养的成本和人力过于繁重。为了克服这些缺点，我们开发了一种新的方法，通过使用骨髓细胞来创建组织工程血管移植物，这种方法可以很容易地获得并立即使用，而无需细胞培养。（V组：静脉细胞培养组，B组：骨髓细胞未培养组，B组：骨髓细胞未培养组）; C组为未接种细胞的移植物（对照组）。结果：在组织学检查中，V组和B组可见一层规则的Masson染色的胶原纤维，一层规则的Ⅷ因子染色的内皮细胞和抗α-平滑肌细胞免疫反应阳性细胞，与天然血管组织相似，而C组未见这种染色的规则衬里。C组中的4-羟脯氨酸测定显示出显著低于V组和B组或天然组织中的水平（P <0.05）。C组的组织工程血管的DNA含量高于V组和B组或自体组织。结论：在组织工程血管的构建中，由于骨髓细胞可以直接使用，不需要培养，因此骨髓细胞的方法似乎是有用的，并且比使用血管细胞的方法更上级。
Objective: To overcome the shortcomings of current vascular grafts, tissue-engineering methods have been applied to cardiovascular regions. We previously reported the creation of a tissue-engineered vascular graft by using vascular mixed cells. However, the cost and manpower for harvesting and culturing the cells was too burdensome. To overcome these drawbacks, we have developed a new method for creating a tissue-engineered vascular graft by using bone marrow cells, which can be obtained easily and used immediately, without cell culture.Methods: Biodegradable polymers seeded with different types of cells (group V, cultured venous cells; group B, bone marrow cells without culture; and group C, non-cell-seeded graft [as control]) were implanted into the inferior venae cavae of dogs. The grafts were explanted at 4 weeks and assessed histologically and biochemically.Results: In the histologic examination, a regular layer of Masson-staining collagen fiber and a layer of factor VIII-stained endothelial and anti-a-smooth muscle cell antigen-immunoreactive cells stained in groups V and B like native vascular tissue, whereas no such stained regular lining was detected in group C. A 4-hydroxyproline assay in group C showed significantly lower levels than in groups V and B or native tissue (P < .05). The DNA content of the tissue-engineered vascular graft tended to be higher in group C than in groups V and B or in native tissue.Conclusions: In the creation of tissue-engineered vascular grafts, the method of using bone marrow cells seems to be useful and superior to that of using vascular cells because bone marrow cells can be used directly, without culture.