Improved method of purifying some radiolabeled glycopeptide hormones.

Improved method of purifying some radiolabeled glycopeptide hormones.
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纯化某些放射性标记糖肽激素的改进方法。

DOI:
10.1093/clinchem/25.1.163
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发表时间:
1979
期刊:
影响因子:
9.3
通讯作者:
W. Odell
W. Odell
中科院分区:
医学1区
文献类型:
--
作者:
N. Patritti;Y. Yoshimoto;A. Wolfsen;W. Odell

文献摘要

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我们描述了一种简化的方法纯化放射性标记的糖肽激素(促甲状腺激素,促黄体激素,促卵泡激素,和人绒毛膜促性腺激素),这导致在95%以上的免疫反应性的制剂。该方法是基于竞争性洗脱柱的植物凝集素,刀豆球蛋白A,并适用于糖肽激素来自几个物种。
We describe a simplified method for purifying radiolabeled glycopeptide hormones (thyrotropin, lutropin, follitropin, and human choriogonadotropin), which results in preparations of over 95% immunoreactivity. This method is based on competitive elution from columns of the plant lectin, concanavalin A, and is applicable to glycopeptide hormones derived from several species.