Imaging Olfactory Learning-Induced Plasticity in Vivo in the Drosophila Brain.
Imaging Olfactory Learning-Induced Plasticity in Vivo in the Drosophila Brain.
复制标题
果蝇大脑体内嗅觉学习诱导的可塑性成像。
DOI：
10.1101/pdb.prot108135
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发表时间：
2023
影响因子：
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通讯作者：
Tomchik,SethM
中科院分区：
文献类型：
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作者：
Boto,Tamara;Tomchik,SethM
在果蝇脑活动的体内成像允许解剖许多类型的生物学上重要的神经元事件。一个共同的范例涉及成像神经元钙瞬变，往往在响应感官刺激.这些Ca2+瞬变与神经元的峰电位活动相关，从而产生电压敏感性Ca2+内流。此外，还有一系列膜电压和其他信号分子（如第二信使信号级联酶和神经递质）的基因编码报告基因，使光学进入一系列细胞过程成为可能。此外，复杂的基因表达系统使得能够接近果蝇大脑中几乎任何单个神经元或神经元组。体内成像方法使得能够研究这些过程以及它们在突出的感觉驱动事件（例如嗅觉联想学习）期间如何变化，当动物（苍蝇）被呈现与非条件刺激（厌恶或食欲刺激）配对的气味（条件刺激）并形成这种配对的联想记忆时。通过光学手段观察大脑中的神经元活动，可以了解联想记忆形成后学习诱导的可塑性，剖析记忆形成、维持和回忆的机制。
In vivo imaging of brain activity in Drosophila allows the dissection of numerous types of biologically important neuronal events. A common paradigm involves imaging neuronal Ca 2+ transients, often in response to sensory stimuli. These Ca 2+ transients correlate with neuronal spiking activity, which generates voltage-sensitive Ca 2+ influx. In addition, there is a range of genetically encoded reporters of membrane voltage and of other signaling molecules, such as second-messenger signaling cascade enzymes and neurotransmitters, enabling optical access to a range of cellular processes. Moreover, sophisticated gene expression systems enable access to virtually any single neuron or neuronal group in the fly brain. The in vivo imaging approach enables the study of these processes and how they change during salient sensory-driven events such as olfactory associative learning, when an animal (fly) is presented an odor (a conditioned stimulus) paired with an unconditioned stimulus (an aversive or appetitive stimulus) and forms an associative memory of this pairing. Optical access to neuronal events in the brain allows one to image learning-induced plasticity following the formation of associative memory, dissecting the mechanisms of memory formation, maintenance, and recall.