Effects of immunization with Cryptococcus neoformans cells or cryptococcal culture filtrate antigen on direct anticryptococcal activities of murine T lymphocytes.
Effects of immunization with Cryptococcus neoformans cells or cryptococcal culture filtrate antigen on direct anticryptococcal activities of murine T lymphocytes.
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新型隐球菌细胞或隐球菌培养滤过抗原免疫对小鼠T淋巴细胞直接抗隐球菌活性的影响。
DOI：
10.1128/iai.63.5.1645-1651.1995
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发表时间：
1995
影响因子：
3.1
通讯作者：
Murphy,JW
中科院分区：
文献类型：
--
作者：
Muth,SM;Murphy,JW
用完整的新型隐球菌细胞或隐球菌培养滤液抗原（CneF）免疫CBA/J小鼠可诱导抗隐球菌迟发型超敏反应。最近，它已被证明，两个表型不同的T细胞群体是负责在小鼠免疫与完整的隐球菌细胞的迟发型超敏反应，而只有一个这些群体是存在于小鼠免疫与可溶性隐球菌抗原在完全弗氏佐剂（CFA）。本研究的目的是确定是否发生差异的直接抗隐球菌活性之间的T-淋巴细胞富集的人口与完整的活的或死的隐球菌细胞免疫的小鼠和类似的细胞群免疫的小鼠的可溶性隐球菌培养滤液抗原，CneF，在CFA乳化。与C.新生隐球菌和隐球菌生长抑制的百分比在体外更大的T-淋巴细胞富集的人口从小鼠亚致死感染C。与来自用CneF-CFA免疫的小鼠的淋巴细胞群相比，来自在CFA存在或不存在下用完整的热灭活隐球菌细胞免疫的小鼠的淋巴细胞群的免疫效果更好。热灭活的C.血清型A、B、C或D的新生儿细胞以及用无囊C的类似制剂免疫。neoformans突变体，但不能通过用从任何一种C.新形式分离物。这些数据表明，可溶性隐球菌培养滤液抗原不诱导与完整隐球菌细胞相同的功能性T淋巴细胞阵列。
Immunizing CBA/J mice with intact Cryptococcus neoformans cells or with a cryptococcal culture filtrate antigen (CneF) induces an anticryptococcal delayed-type hypersensitivity response. Recently, it has been shown that two phenotypically different T-cell populations are responsible for delayed-type hypersensitivity reactivity in mice immunized with intact cryptococcal cells, whereas only one of those populations is present in mice immunized with soluble cryptococcal antigens in complete Freund's adjuvant (CFA). The purpose of this study was to determine if differences occur with regard to direct anticryptococcal activity between T-lymphocyte-enriched populations from mice immunized with intact viable or dead cryptococcal cells and similar cell populations from mice immunized with the soluble cryptococcal culture filtrate antigen, CneF, emulsified in CFA. The percentage of lymphocytes which form conjugates with C. neoformans and the percentage of cryptococcal growth inhibition in vitro are greater with T-lymphocyte-enriched populations from mice sublethally infected with C. neoformans or from mice immunized with intact heat-killed cryptococcal cells in the presence or absence of CFA than with lymphocyte populations from mice immunized with CneF-CFA. Enhanced anticryptococcal activity of T lymphocytes could be induced by immunizing mice with heat-killed C. neoformans cells of serotype A, B, C, or D as well as by immunizing with a similar preparation of an acapsular C. neoformans mutant but not by immunizing with CFA emulsified with CneF prepared from any one of the C. neoformans isolates. These data indicate that the soluble cryptococcal culture filtrate antigens do not induce the same array of functional T lymphocytes as whole cryptococcal cells.