Adsorption of Saliva Related Protein on Denture Materials: An X-Ray Photoelectron Spectroscopy and Quartz Crystal Microbalance Study
Adsorption of Saliva Related Protein on Denture Materials: An X-Ray Photoelectron Spectroscopy and Quartz Crystal Microbalance Study
复制标题
唾液相关蛋白在义齿材料上的吸附：X 射线光电子能谱和石英晶体微天平研究
DOI：
10.1155/2016/5478326
复制
发表时间：
2016
影响因子：
--
通讯作者：
J. Okazaki
中科院分区：
文献类型：
--
作者：
A. Miyake;S. Komasa;Y. Hashimoto;Y. Komasa;J. Okazaki
本研究的目的是用QCM和X射线光电子能谱(XPS)研究不同类型的牛唾液蛋白在PMMA和TiQCM传感器上的吸附行为的差异。通过扫描电子显微镜、X射线光电子能谱和接触角的研究，确定了QCM表面的化学成分和表面润湿性。我们讨论了每个传感器的质量，并通过研究QCM传感器与蛋白质、白蛋白和粘蛋白(一种与唾液相关的蛋白质)的结合来评估高频QCM传感器的潜力。扫描电子显微镜图像显示表面相对均匀，表面粗糙度为纳米级。涂覆在传感器上的薄膜的XPS测量光谱与PMMA和钛的特征光谱的结合能相似。此外，PMMA QCM传感器上唾液相关蛋白的含量高于钛和Au QCM传感器。蛋白质吸附的差异可能与每种材料的润湿性有关。PMMA和TiQCM传感器是研究白蛋白和粘蛋白在义齿表面吸附和解吸的有用工具。
The aim of this study was to evaluate the difference in the adsorption behavior of different types of bovine salivary proteins on the PMMA and Ti QCM sensors are fabricated by spin-coating and sputtering onto bare QCM sensors by using QCM and X-ray photoelectron spectroscopy (XPS). SPM, XPS, and contact angle investigations were carried out to determine the chemical composition and surface wettability of the QCM surface. We discuss the quality of each sensor and evaluate the potential of the high-frequency QCM sensors by investigating the binding between the QCM sensor and the proteins albumin and mucin (a salivary-related protein). The SPM image showed a relatively homogeneous surface with nano-order roughness. The XPS survey spectra of the thin films coated on the sensors were similar to the binding energy of the characteristic spectra of PMMA and Ti. Additionally, the amount of salivary-related protein on the PMMA QCM sensor was higher than those on the Ti and Au QCM sensors. The difference of protein adsorption is proposed to be related to the wettability of each material. The PMMA and Ti QCM sensors are useful tools to study the adsorption and desorption of albumin and mucin on denture surfaces.
石英晶体微天平技术将胎牛血清蛋白吸附到羟基磷灰石纳米晶上
DOI：
--
发表时间：
2009
期刊：
Key Engineering Materials
影响因子：
--
作者：
Tagaya M;Ikoma T;Takemura T;Okuda M;Hanagata N;Yoshioka T;Chakarov D;Kasemo B;Tanaka J
通讯作者：
Tanaka J
抗菌肽组氨酸5在PMMA上的吸附行为
DOI：
--
发表时间：
2006
期刊：
J Biomed Mater Res 77B-1
影响因子：
--
作者：
Yoshinari M;Kato T;Matsuzaka K;Hayakawa T;Inoue T;Oda Y;Okuda K;Shimono M
通讯作者：
Shimono M