Uniaxially Aligned Nanofibrous Cylinders by Electrospinning
Uniaxially Aligned Nanofibrous Cylinders by Electrospinning
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DOI：
10.1021/am301803b
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发表时间：
2012-09-01
影响因子：
9.5
通讯作者：
Zhang, Miqin
中科院分区：
文献类型：
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作者：
Jana, Soumen;Cooper, Ashleigh;Zhang, Miqin
由于其各向异性和高表面积与体积比所提供的独特物理化学性质，对齐纳米纤维在生物医学工程、电子学和储能系统中的应用引起了越来越多的兴趣。然而，将对齐的纳米纤维直接制造或组装成具有实际适用尺寸的 3 维独立结构提出了巨大的挑战。我们报告了一种简单的方法来制造对齐的纳米纤维圆柱体，这是一种广泛使用的几何形式，通过在一对单轴分开的针电极之间的间隙静电纺丝对齐的纳米纤维。通过这种方法，可以很容易地生产出直径为几毫米、长度为几厘米的圆柱形纳米纤维结构。该方法的多功能性通过几种常用的聚合物和陶瓷材料得到了证明，包括聚己内酯（PCL）、壳聚糖/PCL、聚偏二氟乙烯和二氧化钛。对于组织工程中的模型应用，骨骼肌细胞在纳米纤维圆柱体上培养，其沿纳米纤维方向有效地产生高度排列且密集的肌管，有利于肌肉组织再生。具有高度的结构完整性和稳定性，它们可以直接集成到设备中或作为独立结构植入体内，无需基材的支持，从而提高了排列纳米纤维的便携性、效率和适用性。
Aligned nanofibers have drawn increasing interest for applications in biomedical engineering, electronics, and energy storage systems owing to the unique physicochemical properties provided by their anisotropy and high surface-to-volume ratio. Nevertheless, direct fabrication or assembly of aligned nanofibers into a 3-dimensional standalone construct with practically applicable dimensions presents an enormous challenge. We report a facile method to fabricate aligned nanofibrous cylinders, a widely used geometric form, by electrospinning aligned nanofibers across the gap between a pair of pin electrodes placed apart uniaxially. With this approach, cylindrical nanofibrous constructs of several millimeters in diameter and several centimeters in length can be readily produced. The versatility of the approach was demonstrated with several commonly used polymeric and ceramic materials, including polycaprolactone (PCL), chitosan/PCL, polyvinylidene fluoride, and titania. For a model application in tissue engineering, skeletal muscle cells were cultured on nanofibrous cylinders, which effectively produced highly aligned and densely populated myotubes along the nanofiber orientation, favorable for muscle tissue regeneration. With high structural integrity and stability, these can be directly integrated into devices or implanted in vivo as a standalone construct without the support of a substrate, thus increasing the portability, efficiency, and applicability of aligned nanofibers.