Artificial Fusion Protein to Facilitate Calcium Carbonate Mineralization on Insoluble Polysaccharide for Efficient Biocementation
Artificial Fusion Protein to Facilitate Calcium Carbonate Mineralization on Insoluble Polysaccharide for Efficient Biocementation
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DOI：
10.1021/acssuschemeng.1c03730
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发表时间：
2021-08
影响因子：
8.4
通讯作者：
T. K. Nawarathna;K. Nakashima;Tetsuya Kawabe;W. Mwandira;Kiyofumi Kurumisawa;S. Kawasaki
中科院分区：
文献类型：
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作者：
T. K. Nawarathna;K. Nakashima;Tetsuya Kawabe;W. Mwandira;Kiyofumi Kurumisawa;S. Kawasaki
生物矿化是生物体中矿物质形成的过程。与非生物矿物相比，生物矿物可以定义为具有优异物理和光学性质的有机-无机杂化材料。在当前的研究中，开发了一种模仿小龙虾外壳的人工蛋白质，由碳酸钙、甲壳质和蛋白质组成，通过碳酸钙在甲壳质基质上的沉淀来促进有机-无机杂化材料的形成。通过将短序列方解石结合肽（CaBP）引入几丁质结合域（ChBD）中构建融合蛋白（CaBP-ChBD）。通过酶促尿素水解进行的碳酸钙沉淀实验表明，通过添加 CaBP-ChBD 可以显着增加 CaCO3 的形成。此外，CaCO3 有效沉积在用 CaBP-ChBD 修饰的甲壳素颗粒上。最有趣的是，CaBP-ChBD 将在生物水泥技术过程中更有效、更可持续地提高砂固化性能。所开发的重组蛋白可用于工程应用的有机-无机绿色材料的可持续生产。
Biomineralization is a process of mineral formation in living organisms. Compared with nonbiogenic minerals, biominerals can be defined as organic–inorganic hybrid materials that have excellent physical and optical properties. In the current study, an artificial protein mimicking the outer shell of crayfish, composed of CaCO3, chitin, and proteins, was developed to facilitate organic–inorganic hybrid material formation by precipitation of calcium carbonate on the chitin matrix. The fusion protein (CaBP-ChBD) was constructed by introducing a short-sequence calcite-binding peptide (CaBP) into the chitin-binding domain (ChBD). Calcium carbonate precipitation experiments by enzymatic urea hydrolysis revealed that a significant increase in the CaCO3formation was achieved by adding CaBP-ChBD. Also, CaCO3was efficiently deposited on chitin particles decorated with CaBP-ChBD. Most interestingly, CaBP-ChBD would improve the performance in sand solidification more efficiently and sustainably in the process of biocementation technique. The developed recombinant protein could be used for the sustainable production of organic–inorganic green materials for engineering applications.