Uptake and incorporation of amino acids by the preimplantation mouse embryo.
Uptake and incorporation of amino acids by the preimplantation mouse embryo.
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植入前小鼠胚胎对氨基酸的吸收和掺入。
DOI：
10.1530/jrf.0.0270329
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发表时间：
1971
期刊：
影响因子：
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通讯作者：
R. Brinster
中科院分区：
文献类型：
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作者：
R. Brinster
测定了谷氨酰胺和天冬酰胺以及九种氨基酸的吸收和掺入，这些氨基酸在体外培养实验中已被证明对植入前小鼠胚胎的发育很重要。一般来说，从单细胞到桑葚期，氨基酸的掺入增加了十倍，摄取增加了五倍。掺入和摄取的最大变化发生在大约8个细胞的发育阶段。与其他氨基酸相比，亮氨酸和谷氨酰胺被吸收得更多。在一细胞期和二细胞期之间，亮氨酸碳进入胚胎的速率为0\m=。桑葚胚至囊胚的浓度为2 μ m= 153pmol/胚/小时。一细胞期和二细胞期谷氨酰胺碳的总吸收量为1\m=。桑葚胚与囊胚间距为5 μ m。\ 655 pmol /胚胎/人力资源。小鼠卵子受精对亮氨酸的掺入没有显著影响，对亮氨酸的总吸收只有很小的影响。这与无脊椎动物的研究结果形成鲜明对比，在无脊椎动物中，受精导致氨基酸代谢的各个方面都显着增加。
Uptake and incorporation were determined for glutamine and asparagine as well as for nine amino acids which have been shown by culture experiments in vitro to be important in the development of the preimplantation mouse embryo. In general, there was a tenfold increase in the incorporation and a fivefold increase in uptake of the amino acids from the one-cell to the morula stage. The largest change in both incorporation and uptake occurred at about the eight-cell stage of development. Leucine was incorporated more, and glutamine taken up in larger quantities, than the other amino acids. The rate of incorporation of leucine carbon into the embryo between the oneand two-cell stage was 0\m=.\208and for the morula to blastocyst was 2\m=.\153pmol/embryo/hr. The total uptake of glutamine carbon between the oneand two-cell stage was 1\m=.\768and between the morula and blastocyst was 5\m=.\655 pmol/embryo/hr. Fertilization of the mouse ovum did not significantly affect incorporation of leucine and had only a small effect on total uptake of leucine. This contrasts with the findings in invertebrates where fertilization results in a marked increase in all aspects of amino acid metabolism.