Isolation and Characteristics of Methanosaeta in Paddy Field Soils
Isolation and Characteristics of Methanosaeta in Paddy Field Soils
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DOI：
10.1271/bbb.70.828
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发表时间：
2006-01
期刊：
影响因子：
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通讯作者：
Satomi Mizukami;K. Takeda;S. Akada;T. Fujita
中科院分区：
文献类型：
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作者：
Satomi Mizukami;K. Takeda;S. Akada;T. Fujita
淹水条件下，稻田土壤丝状乙酸产甲烷菌数量为2.0×104 MPN/g干土。它们能够在深层琼脂培养基中形成菌落，但不能在卷管中形成菌落。从日本金城（菌株 K-5）和日本筑波（菌株 T-3）分离的丝状利用醋酸盐的产甲烷菌根据丝长度分为两种类型。一种类型，菌株K-5，形成短链，通过弱摇动很容易分散。另一种类型菌株T-3形成长链，形成棉花状絮凝物，弱摇动不会分散。它们的细胞膜外侧有由一对相邻膜组成的鞘。菌株T-3和K-5与Methanosaeta concilii菌株Opfikon的16S rRNA基因相似度分别为100%和99.5%。日本其他地区的稻田土壤中也分离出丝状醋酸盐产甲烷菌。我们的结果表明，甲烷菌在稻田土壤中普遍存在，并且在乙酸盐生产甲烷中发挥着重要作用。
The population of filamentous acetate-utilizing methanogens in paddy field soils was 2.0×104 MPN/g dry soil in the submerged condition. They were able to form colonies in a deep agar medium, but not in a roll tube. Filamentous acetate-utilizing methanogens isolated from Kanagi, Japan (strain K-5) and Tsukuba, Japan (strain T-3) were divided into two types based on length of filaments. One type, strain K-5, formed a short chain which was dispersed easily by weak shaking. The other type, strain T-3, formed a long chain, which formed cotton-like flocs and was not dispersed by weak shaking. They had sheaths composed of a pair of adjacent membranes on the outside of the cell membranes. The 16S rRNA gene similarities of strain T-3 and K-5 to Methanosaeta concilii strain Opfikon were 100% and 99.5% respectively. Filamentous acetate-utilizing methanogens were also isolated from paddy field soils in various other regions of Japan. Our results suggest that Methanosaeta is universal in paddy soils and that it plays an important role in methane production from acetate.