DNA-binding protein nanotubes: Learning from nature's nanotech examples
DNA-binding protein nanotubes: Learning from nature's nanotech examples
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DOI：
10.1021/nl048942f
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发表时间：
2004-10-01
期刊：
影响因子：
10.8
通讯作者：
Irvin, RT
中科院分区：
文献类型：
--
作者：
Audette, GF;van Schalk, EJ;Irvin, RT
细菌的皮利是由蛋白质亚单位组成的纳米纤维。在这里，我们报告的蛋白质纳米管的控制装配工程铜绿假单胞菌IV型菌毛蛋白单体。纳米管的长度可达100 μ m，外径约为6 nm，预计内径约为2 nm。蛋白质纳米管的形成似乎是通过在菌毛蛋白单体中的疏水物引发的构象转变进行的，然后菌毛蛋白单体自缔合以形成薄的线性细丝，所述细丝聚结以形成长的蛋白质纳米管。蛋白质纳米管对纳米技术非常有吸引力，因为蛋白质工程赋予了对机械和化学性质的前所未有的控制。此外，像IV型皮利一样，我们的纳米管结合DNA，进一步扩大了它们在纳米技术应用中的吸引力。
Bacterial pili are nanofibers made of protein subunits. Here we report the controlled assembly of protein nanotubes from an engineered Pseudomonas aeruginosa type IV pilin monomer. The nanotubes are up to 100 mum long with an outer diameter of similar to6 nm and a predicted inner diameter of similar to2 nm. Protein nanotube formation appears to proceed through a hydrophobe-initiated conformational shift in the pilin monomer, which then self-associates to form thin linear filaments that coalesce to form long protein nanotubes. Protein nanotubes are highly attractive for nanotechnology, as protein engineering confers unprecedented control over mechanical and chemical properties. Moreover, like type IV pili, our nanotubes bind DNA, further broadening their appeal in nanotechnological applications.