The effect of anti-sandfly saliva antibodies on Phlebotomus argentipes and Leishmania donovani
The effect of anti-sandfly saliva antibodies on Phlebotomus argentipes and Leishmania donovani
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DOI：
10.1016/s0020-7519(97)00152-5
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发表时间：
1998-02-01
影响因子：
4
通讯作者：
Mukhopadhyay, J
中科院分区：
文献类型：
--
作者：
Ghosh, KN;Mukhopadhyay, J
进行了一项研究，以查明白蛉（白蛉）反复叮咬对其宿主和媒介本身的影响。这项研究还旨在发现免疫血清对由病媒自然传播的多诺瓦利什曼原虫的影响。饲养白蛉的仓鼠对白蛉唾液腺分泌有良好的抗体效价，说明白蛉唾液腺分泌具有免疫原性。结果还表明，在随后的叮咬过程中，随着死亡率的增加，雌性的摄食吸引力（以充血百分比表示）逐渐降低。采用人工膜饲养法饲喂免疫血清时，蝇类死亡率相似。当白蛉同时被喂食免疫血清和血寄生虫（L. donovani）悬浮液时，除了媒介数量的大量减少外，还抑制了肠道发育，同时减少了存活雌性白蛉体内寄生虫的迁移。这些结果表明，抗白蛉唾液免疫血清可能与白蛉唾液腺各自的抗原呈递位点结合，从而导致白蛉死亡。寄生虫向前运动受到抑制的可能解释是，由白蛉唾液介导的寄生虫对食道的吸引被抗唾液抗体抑制了。抗白蛉唾液抗体作为媒介控制和阻断利什曼病传播的重要工具已被指出。(C) 1998澳大利亚寄生虫学学会。Elsevier Science Ltd.出版。
A study was undertaken to find the effect of repeated bites of the sandfly, Phlebotomus argentipes, on its host as well as on the vector itself. The study also aimed to find the effect of the immune serum on the parasite, Leishmania donovani, naturally transmitted by the vector. The hamster which was exposed to sandfly feeding showed good antibody titre against the sandfly salivary-gland secretion, which indicates that the salivary-gland secretion is immunogenic in nature. The result also revealed that the feeding attraction of the females, which has been expressed as the percentage of engorgement, gradually decreased as the mortality rate increased during the subsequent bites. Similar mortality rate was observed when the flies were fed with the immune sera through an artificial membrane feeding method. When the sandflies were fed both with the immune sera and the blood-parasite (L. donovani) suspension, in addition to the major loss of the number of vectors, there was an inhibition of development in the gut and a concomitant reduction in the migration of the parasite in the surviving females. These results indicate that the anti-sandfly saliva immune sera probably bind with the respective antigen-presenting sites of the sandfly salivary gland and, thus, cause the sandfly death. The possible explanation of the inhibition of the forward movement of the parasites is that the attraction of the parasites to the oesophagus, mediated by the sandfly saliva, is inhibited by the anti-saliva antibodies. The importance of anti-sandfly saliva antibodies as a tool of vector control and also to block the transmission of leishmaniasis has been indicated. (C) 1998 Australian Society for Parasitology. Published by Elsevier Science Ltd.