EFFECTS OF ANDROGENS AND ESTROGENS ON THE VASOPRESSIN AND OXYTOCIN INNERVATION OF THE ADULT-RAT BRAIN
EFFECTS OF ANDROGENS AND ESTROGENS ON THE VASOPRESSIN AND OXYTOCIN INNERVATION OF THE ADULT-RAT BRAIN
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DOI：
10.1016/0006-8993(86)91519-2
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发表时间：
1986-12-10
期刊：
影响因子：
2.9
通讯作者：
VREEBURG, JTM
中科院分区：
文献类型：
--
作者：
DEVRIES, GJ;DUETZ, W;VREEBURG, JTM
最近我们报道，对大鼠进行去势消除了侧隔膜和其他似乎接受来自终纹床核的加压素神经支配的区域的加压素免疫反应性。睾酮治疗可以抵消这种阉割的影响。在本研究中，我们通过用雌二醇 (E) 和/或 5α-二氢睾酮 (DHT) 或睾酮治疗长期去势的大鼠，研究睾酮的这种作用是否取决于其雄激素或雌激素代谢物。然后对大脑进行免疫细胞化学或放射免疫测定。尽管 DHT 完全恢复了精囊的大小，但它并没有增加侧隔膜中的加压素染色。 E确实恢复了原始的纤维密度，但单个纤维的染色比假手术男性的染色更弱。只有同时使用 E 和 DHT 治疗才能完全恢复加压素神经支配。这种模式也反映在放射免疫分析数据中。去势后侧隔膜的加压素含量减少约 90%，但通过睾酮或 E+DHT 治疗完全恢复。然而，单独使用 E 的效果仅为 E+DHT 的一半。这些治疗对隔膜的催产素含量或背侧迷走神经复合体的加压素或催产素含量没有影响。结果表明，E 介导睾酮对外侧隔膜加压素神经支配的大部分影响。 DHT 增强对 E 的反应，但其本身作用不大。
Recently we reported that castration of rats eliminates vasopressin immunoreactivity in the lateral septum and other areas that appear to receive vasopressin innervation from the bed nucleus of the stria terminalis. Testosterone treatment counteracts this effect of castration. In the present study, we investigated whether this action of testosterone depends on its adrogenic or estrogenic metabolites by treating long-term castrated rats with estradiol (E) and/or 5α-dihydrotestosterone (DHT) or testosterone. The brains were then processed for immunocytochemistry or radioimmunoassay. DHT did not increase vasopressin staining in the lateral septum, although it fully restored the size of the seminal vesicles. E did restore the original fiber density, but individual fibers stained more weakly than in sham-operated males. Only treatment with both E and DHT fully restored the vasopressin innervation. This pattern was also reflected in the radioimmunoassay data. The vasopressin content of the lateral septum decreased about 90% after castration but was fully restored by either testosterone orE+DHT treatment. E alone however, was only half as effective asE+DHT. The treatments had no effect on the oxytocin content of the septum, or on the vasopressin or oxytocin content of the dorsal vagal complex. The results suggest that E mediates most of the effects of testosterone on the vasopressin innervation of the lateral septum. DHT enhances the response to E but has little effect on its own.