Platelets are a possible regulator of human endometrial re-epithelialization during menstruation
Platelets are a possible regulator of human endometrial re-epithelialization during menstruation
复制标题
血小板可能是月经期间人类子宫内膜再上皮化的调节者
DOI：
10.1111/aji.12609
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发表时间：
2016
影响因子：
3.6
通讯作者：
Fujiwara H
中科院分区：
文献类型：
--
作者：
Suginami K;Sato Y;Horie A;Matsumoto H;Kyo S;Araki Y;Konishi I;Fujiwara H
问题人类子宫内膜会周期性地分解和再生。据报道，血小板有助于组织重塑过程，我们检查了血小板在子宫内膜再生中的可能参与。研究方法使用人类子宫内膜组织，对整个月经周期中外渗血小板的分布进行了免疫组织化学检查。将人子宫内膜上皮细胞来源的永生化细胞系 EM-E6/E7/hTERT 细胞与血小板共培养，通过贴壁和通透性实验、免疫组化染色和 Western blot 分析研究血小板对 EM-E6/E7/hTERT 细胞上皮化反应的影响。结果免疫组化研究显示月经期间管腔下基质中有大量血小板外渗。阶段。血小板促进 EM-E6/E7/hTERT 细胞与基质胶的附着，同时促进粘着斑激酶的磷酸化。它们还与 E-钙粘蛋白表达同时促进 EM-E6/E7/hTERT 细胞之间的细胞间接触。结论这些结果表明血小板可能参与子宫内膜上皮再上皮化过程。
ProblemThe human endometrium periodically breaks down and regenerates. As platelets have been reported to contribute to the tissue remodeling process, we examined the possible involvement of platelets in endometrial regeneration.Method of studyThe distribution of extravasating platelets throughout the menstrual cycle was immunohistochemically examined using human endometrial tissues. EM‐E6/E7/hTERT cells, a human endometrial epithelial cell‐derived immortalized cell line, were co‐cultured with platelets, and the effects of platelets on the epithelialization response of EM‐E6/E7/hTERT cells were investigated by attachment and permeability assays, immunohistochemical staining, and Western blot analysis.ResultsImmunohistochemical study showed numerous extravasated platelets in the subluminar stroma during the menstrual phase. The platelets promoted the cell‐to‐matrigel attachment of EM‐E6/E7/hTERT cells concomitantly with the phosphorylation of focal adhesion kinase. They also promoted cell‐to‐cell contact among EM‐E6/E7/hTERT cells in parallel with E‐cadherin expression.ConclusionThese results indicate the possible involvement of platelets in the endometrial epithelial re‐epithelialization process.