Preparation and evaluation of molecularly imprinted polymeric microspheres by aqueous suspension polymerization for use as a high-performance liquid chromatography stationary phase
Preparation and evaluation of molecularly imprinted polymeric microspheres by aqueous suspension polymerization for use as a high-performance liquid chromatography stationary phase
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DOI：
10.1016/s0003-2670(01)01115-1
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发表时间：
2001-08-31
影响因子：
6.2
通讯作者：
He, XW
中科院分区：
文献类型：
--
作者：
Lai, JP;Lu, XY;He, XW
以4-氨基吡啶为印迹分子，采用悬浮聚合法制备了分子印迹聚合物微球。MIPM装入不锈钢柱(250 Mm)。X 4.6毫米内径)用于4-氨基吡啶和2-氨基吡啶的选择性分离和定量测定。考察了不同pH缓冲液(醋酸盐溶液)调节的流动相对容量因子的影响。结果表明，4-氨基吡啶的容量因数(k‘)随流动相pH的升高而增大，而2-氨基吡啶的容量因数(k’)变化不大。研究表明，MIPM固定相和流动相对底物的容量因子均有影响。在低pH值(pH<5.5)流动相中，4-氨基吡啶优先于2-氨基吡啶洗脱，容量因子(k‘)较低。但在高pH(pH&Gt；5.5)流动相中，模板分子的容量因子(k‘)远高于非印迹分子。因此，将4-氨基吡啶印迹微球用于4-氨基吡啶和2-氨基吡啶的分离和同时测定。峰面积对底物浓度的响应与R>；0.999呈良好的线性关系。该方法可通过改变流动相的pH值，同时分离和测定价格昂贵或得不到或物化性质相近的多组分同系物或异构体。(C)2001 Elsevier Science B.V.保留所有权利。
Molecularly imprinted polymeric microspheres (MIPMs) were prepared using 4-aminopyridine as imprinting molecule by aqueous suspension polymerization. The MIPMs were packed into stainless steel column (250 mm. x 4.6 mm i.d.) for selective separation and quantitative determination of 4-aminopyridine and 2-aminopyridine. The influence of mobile phases adjusted with different pH buffers (acetate solution) on capacity factors were investigated. The results showed that the capacity factor (k ') for 4-aminopyridine increased with increasing pH of mobile phase while that of 2-aminopyridine was subtly changed. The studies showed that the capacity factors for substrates were affected by both of MIPM stationary phase and mobile phase. In low pH (pH less than or equal to 5.5) mobile phase, the 4-aminopyridine was eluted prior to 2-aminopyridine and its capacity factor (k ') was lower. In high pH (pH > 5.5) mobile phase, however, the capacity factor (k ') for template molecule exhibited far higher than that of non-imprinting molecule. Therefore, the imprinted microspheres against 4-aminopyridine were used to separate and simultaneous determinate 4-aminopyridine and 2-aminopyridine. The responses of peak areas versus concentrations of substrates present good linearity with R > 0.999. This method may be used to separate and simultaneous determinate multicomponental homologous compounds or isomers, which are very expensive or unavailable or whose physicochemical properties are very similar, by changing the pH of mobile phases. (C) 2001 Elsevier Science B.V. All rights reserved.