DNA metabarcoding allows non-invasive identification of arthropod prey provisioned to nestling Rufous hummingbirds (Selasphorus rufus)
DNA metabarcoding allows non-invasive identification of arthropod prey provisioned to nestling Rufous hummingbirds (Selasphorus rufus)
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DOI：
10.7717/peerj.6596
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发表时间：
2019-03-05
期刊：
影响因子：
2.7
通讯作者：
Mora, Jonathan A.
中科院分区：
文献类型：
--
作者：
Mora, Alison J.;Prosse, Sean W. J.;Mora, Jonathan A.
蜂鸟从花蜜、树液和蜜露中摄取糖分，并从节肢动物中获取蛋白质、脂肪和矿物质。到目前为止，蜂鸟饮食中节肢动物类群的身份已经通过观察觅食或检查消化道内容物进行了研究。直接检查雏鸟的供应增加了额外的复杂性的干扰年轻和母亲。在这里，我们表明，节肢动物的食物提供给棕褐色蜂鸟（Selasphorus rufus）雏鸟可以确定一个安全和非侵入性的协议，使用下一代测序（NGS）的DNA从雏鸟粪便颗粒收集后羽翼未丰。我们发现，女性在南部温哥华岛（不列颠哥伦比亚省，加拿大）提供雏鸟与广泛的节肢动物类群。所研究的样品包含3个纲、8个目、48个科和87个属，其中1至15个科在一个颗粒中被鉴定。软体双翅目被发现最频繁，具有最高的相对丰度，硬体的猎物项目几乎没有所有的样品。分类群的显着差异，发现在季节和年份之间，表明多年的采样时，定义的猎物谱的重要性。
Hummingbirds consume sugars from nectar, sap and honeydew, and obtain protein, fat and minerals from arthropods. To date, the identity of arthropod taxa in hummingbird diets has been investigated by observation of foraging or examination of alimentary tract contents. Direct examination of nestling provisioning adds the extra complication of disturbance to the young and mother. Here, we show that arthropod food items provisioned to Rufous hummingbird (Selasphorus rufus) nestlings can be identified by a safe and non-invasive protocol using next-generation sequencing (NGS) of DNA from nestling fecal pellets collected post-fledging. We found that females on southern Vancouver Island (British Columbia, Canada) provisioned nestlings with a wide range of arthropod taxa. The samples examined contained three Classes, eight Orders, 48 Families, and 87 Genera, with from one to 15 Families being identified in a single pellet. Soft-bodied Dipterans were found most frequently and had the highest relative abundance; hard-bodied prey items were absent from almost all samples. Substantial differences in taxa were found within season and between years, indicating the importance of multi-year sampling when defining a prey spectrum.