A Role for the Internal Repeat of the Kaposi's Sarcoma-Associated Herpesvirus Latent Nuclear Antigen in the Persistence of an Episomal Viral Genome
A Role for the Internal Repeat of the Kaposi's Sarcoma-Associated Herpesvirus Latent Nuclear Antigen in the Persistence of an Episomal Viral Genome
复制标题
DOI：
10.1128/jvi.06029-11
复制
发表时间：
2012-02-01
影响因子：
5.4
通讯作者：
Schulz, Thomas F.
中科院分区：
文献类型：
--
作者：
Alkharsah, Khaled R.;Schulz, Thomas F.
卡波济肉瘤相关疱疹病毒（KSHV）的潜伏核抗原（拉娜）是复制和分配潜伏病毒基因组所必需的。它包含一个扩展的内部重复（IR）区域，其功能仅不完全了解。我们构建了缺失拉娜（KSHV-Delta拉娜）或拉娜的IR区（KSHV-拉娜Delta 329-931）的KSHV基因组。尽管仍然能够复制含有潜在复制起点的质粒，但拉娜Delta 329-931不支持建立含有KSHV基因组的稳定细胞系。这些发现表明拉娜IR在KSHV游离型维持中的作用，而不需要复制。
The latent nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) is required for the replication and partitioning of latent viral genomes. It contains an extended internal repeat (IR) region whose function is only incompletely understood. We constructed KSHV genomes lacking either LANA (KSHV-Delta LANA) or the IR region of LANA (KSHV-LANA Delta 329-931). Although still capable of replicating a plasmid containing a latent origin of replication, LANA Delta 329-931 does not support the establishment of stable cell lines containing a KSHV genome. These findings suggest a role for the LANA IR in KSHV episomal maintenance without its being required for replication.