MiR-202-5p Promotes M2 Polarization in Allergic Rhinitis by Targeting MATN2.
MiR-202-5p Promotes M2 Polarization in Allergic Rhinitis by Targeting MATN2.
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MiR-202-5p 通过靶向 MATN2 促进过敏性鼻炎中的 M2 极化。
DOI：
10.1159/000493803
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发表时间：
2019
影响因子：
2.8
通讯作者：
Liu Dawei
中科院分区：
文献类型：
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作者：
Wang Li;Liu Xueyan;Song Xicheng;Dong Lei;Liu Dawei
本研究的目的是探讨MiR-202-5p对过敏性鼻炎中巨噬细胞的影响。方法选取30例变应性鼻炎患者和10例健康对照者。用鼻灌洗法收集粘膜组织和粘液源性巨噬细胞。采用流式细胞术检测和分选2型巨噬细胞，采用qRT-PCR和Western blot检测miR-202-5p和MATN2的表达。荧光素酶报告基因测定和生物素- rna下拉证实miR-202-5p与MATN2的直接结合。进一步过表达MATN2以检测其对M2极化的影响。结果smir -202-5p在变应性鼻炎粘膜组织和巨噬细胞中表达较高，具有促进M2极化的作用。MATN2可被miR-202-5p直接靶向，并可逆转miR-202-5p介导的变应性鼻炎M2表型极化。结论smir -202-5p可靶向MATN2诱导M2极化参与变应性鼻炎。
BackgroundThe aim of this study was to investigate the effect of MiR-202-5p on macrophages involved in allergic rhinitis.MethodsThirty allergic rhinitis patients and 10 healthy controls were recruited. Nasal lavage was utilized to collect mucosal tissues and mucus-derived macrophages. Flow cytometry was used to detect and sort type 2 macrophages, and qRT-PCR and Western blot were adopted to determine the expression of miR-202-5p and MATN2. Luciferase reporter assay and biotin-RNA pulldown were performed to testify the direct binding of miR-202-5p with MATN2. MATN2 was further overexpressed to test its effect on M2 polarization.ResultsmiR-202-5p, which can promote M2 polarization, was highly expressed in allergic rhinitis mucosal tissues and macrophages compared with the healthy controls. MATN2 could be directly targeted by miR-202-5p and can reverse miR-202-5p-mediated M2 phenotype polarization in allergic rhinitis.ConclusionsmiR-202-5p could target MATN2 to induce M2 polarization involved in allergic rhinitis.