Genome-wide analysis of ionotropic receptor gene repertoire in Lepidoptera with an emphasis on its functions of Helicoverpa armigera
Genome-wide analysis of ionotropic receptor gene repertoire in Lepidoptera with an emphasis on its functions of Helicoverpa armigera
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鳞翅目离子型受体基因库的全基因组分析，重点关注棉铃虫的功能
DOI：
10.1016/j.ibmb.2018.05.005
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发表时间：
2018-08-01
影响因子：
3.8
通讯作者：
Anderson, Alisha
中科院分区：
文献类型：
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作者：
Liu, Nai-Yong;Xu, Wei;Anderson, Alisha
嗜离子受体（IR）家族的功能在黑腹果蝇中已经得到了很好的研究，但在鳞翅目中的研究却很有限。在这里，我们对13个月和16只蝴蝶的IR基因库进行了大规模的全基因组分析。结合同源性方法和人工方法，共鉴定出996个候选IR，其中包括31个假基因和825个全长序列，代表了鳞翅目物种中最新的综合注释。鳞翅目IRs的系统发育、表达和序列特征将鳞翅目IRs分为触角IRs （A-IRs）、发散IRs （D-IRs）和鳞翅目特异性IRs （LS-IRs）三个亚科，这与果蝇IRs的情况有所不同。与LS-IRs和D-IRs相比，a - irs成员具有更高的蛋白质同一性，在系统发育上可分为16个同源群，具有基因结构的保守性。对27个同源基团的选择力分析表明，这些鳞翅目ir是在强净化选择（dN/dS）下进化而来的
The functions of the Ionotropic Receptor (IR) family have been well studied in Drosophila melanogaster, but only limited information is available in Lepidoptera. Here, we conducted a large-scale genome-wide analysis of the IR gene repertoire in 13 moths and 16 butterflies. Combining a homology-based approach and manual efforts, totally 996 IR candidates are identified including 31 pseudogenes and 825 full-length sequences, representing the most current comprehensive annotation in lepidopteran species. The phylogeny, expression and sequence characteristics classify Lepidoptera IRs into three sub-families: antennal IRs (A-IRs), divergent IRs (D-IRs) and Lepidoptera-specific IRs (LS-IRs), which is distinct from the case of Drosophila IRs. In comparison to LS-IRs and D-IRs, A-IRs members share a higher degree of protein identity and are distinguished into 16 orthologous groups in the phylogeny, showing conservation of gene structure. Analysis of selective forces on 27 orthologous groups reveals that these lepidopteran IRs have evolved under strong purifying selection (dN/dS