Long non-coding RNA CCAT1 promotes glioma cell proliferation via inhibiting microRNA-410
Long non-coding RNA CCAT1 promotes glioma cell proliferation via inhibiting microRNA-410
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DOI：
10.1016/j.bbrc.2016.10.047
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发表时间：
2016-11-25
影响因子：
3.1
通讯作者：
Zheng, Dong-Lin
中科院分区：
文献类型：
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作者：
Wang, Zhao-Hui;Guo, Xia-Qing;Zheng, Dong-Lin
背景与目的：长链非编码RNA已被证实在多种癌症中发挥关键作用。在本研究中，长链非编码RNA（IncRNA）CCAT 1对胶质瘤细胞增殖的影响及其潜在的mechanism.Methods和结果：Real-time PCR结果显示，IncRNA-CCAT 1的表达显着上调与对照组相比，在胶质瘤癌组织和细胞系。用siRNA-CCAT 1（si-CCAT 1）抑制胶质瘤细胞系U251中CCAT 1的表达后，细胞存活率和集落形成能力下降，细胞周期阻滞于G1期，细胞凋亡增加。如生物信息学软件starbase2.0中所报道的，总共22种microRNA可能被CCAT 1靶向。证实miR-410被si-CCAT 1改变最多。上调U251细胞中CCAT 1表达后，miR-410水平降低。荧光素酶报告基因分析证实CCAT 1靶向miR-410。相关分析显示，胶质瘤癌组织中CCAT 1与miR-410表达呈负相关。结论：lncRNA-CCAT 1通过抑制miR-410而促进胶质瘤细胞增殖，为胶质瘤增殖机制的研究提供了新的思路。(C)2016 Elsevier Inc. All rights reserved.
Background and aim: Long non-coding RNAs have been confirmed to play a critical role in various cancers. In the present study, the effect of long non-coding RNA (IncRNA) CCAT1 on glioma cell proliferation and its potential mechanism were investigated.Methods and results: Real-time PCR results showed that IncRNA-CCAT1 expression was significantly upregulated in glioma cancer tissues and cell lines compared with controls. After inhibiting CCAT1 expression in glioma cell line U251 with siRNA-CCAT1 (si-CCAT1), the cell viability and cell colony formation were decreased, the cell cycle was arrested in G1 phase, and the cell apoptosis was increased. As reported in bioinformatics software starbase2.0, a total of 22 microRNAs were potentially targeted by CCAT1. It was confirmed that miR-410 was altered most by si-CCAT1. After up-regulating CCAT1 expression in U251 cells, miR-410 level was decreased. Luciferase reporter assay confirmed that CCAT1 targeted miR-410. Correlation analysis showed that CCAT1 expression was negatively related to miR-410 expression in glioma cancer tissues. In addition, down-regulation of miR-410 reversed the inhibitory effect of si-CCAT1 on glioma proliferation.Conclusion: These data demonstrated that lncRNA-CCAT1 promoted glioma cell proliferation via inhibiting miR-410, providing a new insight about the pathogenesis of glioma proliferation. (C) 2016 Elsevier Inc. All rights reserved.